slide1
Download
Skip this Video
Download Presentation
VC221: Tomato

Loading in 2 Seconds...

play fullscreen
1 / 27

VC221: Tomato - PowerPoint PPT Presentation


  • 88 Views
  • Uploaded on

VC221: Tomato. sequencing. Key for pachytene chromosome identification. 1st criteria: Chromosome with NOR vs without with: chromosome 2 2nd criteria: Position of the centromere a) Median chromosomes 5, 11, 12 (metacentric) b) Sub median all others (sub-metacentric)

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'VC221: Tomato' - tablita


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide6

Key for pachytene chromosome identification

  • 1st criteria: Chromosome with NOR vs without with: chromosome 2
  • 2nd criteria: Position of the centromere
    • a) Median chromosomes 5, 11, 12 (metacentric)
    • b) Sub median all others (sub-metacentric)
  • 3rd criteria: Short arm heterochromatic regions
    • a) A conspicuous cluster of chromomeres compressed together: chromosomes. 3, 4, 6.
    • b) More than 1 cluster of chromomeres: chromosomes. 1, 7, 10.
slide10

Tomato Mapping Stocks

LA 1182 sy - sf(3) alb - mua (12)

LA1441 coa - c(6) a - hl (11)

LA1443 scf - dgt(1) l -al(8)

LA1444 wd - d(2) af - tf(5)

LA1445 clau-di(4) icn - ag(10)

LA1446 var - not(7) ah - marm(9)

slide17

(based on 6 DNA seq, Adh 2, fw2.2)

S. lycopersicon

Eulycopersicon

(color-fruited)

typ: S. cheesmaniae

min: S. galapagence

S. pimpinellifolium

S. neorickii

S. chemieleskii

Eriopersicon

Green-fruited

S. peruvianum

S. arcanum

S. Corneliomuellerii

S. huayalasense

S. habrochaites

S. pennellii

S. chilense

S. lycopersicoides, S. ochrantum, S. sitiens

slide20

Dimeric

Monomeric

slide21

Esc-pennelli introgression lines

Each stock is homozygous for a single chromosome segment (delineated by RFLP markers) introgressed from L. pennellii, such that the entire wild species genome is represented in a group of 50 lines.

dissection of qtl se2 1 stigma exertion
Dissection of QTL se2.1(stigma exertion
  • Four loci influencing stamen length (three closely linked) were identified.
  • A single locus for style length was identified (the long style allele was shown to be dominant using esc-penn sustitution lines).
  • A single locus for anther dehiscence was also found in the region.

Chen and Tanksley 2004

slide24
Five closely linked genes all contribute to stigma exsertion phenotype.

This may be the remnant of a “co adapted gene complex” where linked genes cooperatively contribute to an adaptation, and are inherited as a single unit.

ad