VC221: Tomato
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VC221: Tomato. sequencing. Key for pachytene chromosome identification. 1st criteria: Chromosome with NOR vs without with: chromosome 2 2nd criteria: Position of the centromere a) Median chromosomes 5, 11, 12 (metacentric) b) Sub median all others (sub-metacentric)

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VC221: Tomato

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Vc221 tomato

VC221: Tomato


Vc221 tomato

sequencing


Vc221 tomato

Key for pachytene chromosome identification

  • 1st criteria: Chromosome with NOR vs without with: chromosome 2

  • 2nd criteria: Position of the centromere

    • a) Median chromosomes 5, 11, 12 (metacentric)

    • b) Sub median all others (sub-metacentric)

  • 3rd criteria: Short arm heterochromatic regions

    • a) A conspicuous cluster of chromomeres compressed together: chromosomes. 3, 4, 6.

    • b) More than 1 cluster of chromomeres: chromosomes. 1, 7, 10.


Vc221 tomato

Tomato Mapping Stocks

LA 1182 sy - sf(3) alb - mua (12)

LA1441 coa - c(6) a - hl (11)

LA1443 scf - dgt(1) l -al(8)

LA1444 wd - d(2) af - tf(5)

LA1445 clau-di(4) icn - ag(10)

LA1446 var - not(7) ah - marm(9)


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Distribution of tomato species

Zuriaga et al 2008


Vc221 tomato

L. cheesmanii minor typicum


Vc221 tomato

(based on 6 DNA seq, Adh 2, fw2.2)

S. lycopersicon

Eulycopersicon

(color-fruited)

typ: S. cheesmaniae

min: S. galapagence

S. pimpinellifolium

S. neorickii

S. chemieleskii

Eriopersicon

Green-fruited

S. peruvianum

S. arcanum

S. Corneliomuellerii

S. huayalasense

S. habrochaites

S. pennellii

S. chilense

S. lycopersicoides, S. ochrantum, S. sitiens


Vc221 tomato

Zuriaga et al 2008


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Dimeric

Monomeric


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Esc-pennelli introgression lines

Each stock is homozygous for a single chromosome segment (delineated by RFLP markers) introgressed from L. pennellii, such that the entire wild species genome is represented in a group of 50 lines.


Dissection of qtl se2 1 stigma exertion

Dissection of QTL se2.1(stigma exertion

  • Four loci influencing stamen length (three closely linked) were identified.

  • A single locus for style length was identified (the long style allele was shown to be dominant using esc-penn sustitution lines).

  • A single locus for anther dehiscence was also found in the region.

Chen and Tanksley 2004


Fine mapping loci contributing to the se2 1 qtl

Fine mapping loci contributing to the se2.1 QTL


Vc221 tomato

Five closely linked genes all contribute to stigma exsertion phenotype.

This may be the remnant of a “co adapted gene complex” where linked genes cooperatively contribute to an adaptation, and are inherited as a single unit.


Vc221 tomato

Tomato Genome Sequencing Project


Vc221 tomato

Tomato SI


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