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ELF-Induced DNA Breaks

ELF-Induced DNA Breaks. Henry Lai Department of Bioengineering University of Washington Seattle, WA USA. DNA Strand breaks -single and double strand breaks -mis-repair cumulative -affect cell functions; can lead to cancer and cell death. COMET ASSAY.

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ELF-Induced DNA Breaks

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  1. ELF-Induced DNA Breaks Henry Lai Department of Bioengineering University of Washington Seattle, WA USA

  2. DNA Strand breaks -single and double strand breaks -mis-repair cumulative -affect cell functions; can lead to cancer and cell death

  3. COMET ASSAY

  4. DNA strand breaks have been reported in different cell types after ELF EMF exposure. Ahuja (99)- human leukocytes Delimaris (06)- human lymphocytes Hong (05)- mouse sperm cells Ivancsit (02, 05)- human fibroblasts and melanocytes Ivancsit (03)-more pronounced effect observed in fibroblasts of older subjects. Jajte (01)- rat lymphocytes Lai and Singh (97, 04)- rat brain cells Li (01)- E. coli Svedenstal (99)- mouse brain cells Wolf (05)- HL-60 and rat fibroblasts Zmyslong (00, 04)- rat lymphocytes

  5. ELF EMF affects DNA repair Robison (02) HL-60 –decrease Chow (00) – E. coli- increase- heat shock protein Schmitz (04)- Mouse brain and kidney cells- increase

  6. ELF EMF genotoxic effects • involve free radicals • Effects blocked by free radical scavengers, enhanced by divalent metals, increase oxidative-damage in molecules • Jaite (01) • Lai and Singh (97) • Li (01) • Lourencini (00) • Yokus (05) • Wolf (05) • Zmyslong (00, 04)

  7. Electromagnetic fields iron OH H2O2 mitochondria Cellular damage THE FENTON REACTION

  8. Intensity and duration of exposure interact. High intensity/short duration = Low intensity/long duration

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