Emf protein analysis flow chart
This presentation is the property of its rightful owner.
Sponsored Links
1 / 1

EMF Protein Analysis Flow Chart PowerPoint PPT Presentation


  • 62 Views
  • Uploaded on
  • Presentation posted in: General

Isolated Protein. Gel protein spot (1D / 2D). Blotted on PVDF membrane. Figure I. EMF Protein Analysis Flow Chart. Biological material (Tissue, Cells, Body fluids). HPLC/ Purification. Gel electrophoresis. Digestion/ cleavage. Digestion/ cleavage. Digestion/ cleavage.

Download Presentation

EMF Protein Analysis Flow Chart

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Emf protein analysis flow chart

Isolated

Protein

Gel

protein spot

(1D / 2D)

Blotted on

PVDF

membrane

Figure I

EMF Protein Analysis Flow Chart

Biological material

(Tissue, Cells, Body fluids)

HPLC/

Purification

Gel electrophoresis

Digestion/

cleavage

Digestion/

cleavage

Digestion/

cleavage

Edman

degradation

(sequence) 500 fmol

Amino Acid

Analysis

1 - 10 pmol

Peptide mixture

Edman

degradation

(sequence)

 500 fmol

Desalting (ZipTip)

or Nanodesalting

Proposed

Addition

HPLC fractionation

Fractionated

peptide

MALDI-TOF

(mass map)

 50 fmol

LC-ESI-MS

(mass map)

1 - 5 pmol

Nano ESI-MS

(mass map)

0.5 - 1 pmol

LC-ESI-MS/MS

(MS scans plus MS/MS scans)

mass map + sequence tag

Q-Tof Ultima

50 - 500 fmol

PSD

MALDI-TOF

(sequence tag)

 100 fmol

Nano

ESI-MS/MS

(sequence tag)

0.5 -1 pmol

Edman

degradation

(sequence)

 300 fmol

PSD

MALDI-TOF

(sequence tag)

 100 fmol

Nano

ESI-MS/MS

(sequence tag)

0.5 - 1 pmol

Database searches

(SEQUEST, Mascot, PROWL, Protein Prospector, etc.)


  • Login