Live Cell Imaging By CLASS Microscopy Rajarshi Choudhury. Microscopy. Why we use microscope? Better magnification Better resolution Determination of ultra structure What are the factors that determine the resolution of a point in microscope?. Different types of microscopy. Light
Confocal light absorption and scattering spectroscopic microscopy (CLASS) monitors organelles in live cells with no exogenous labels
Irving Itzkan, Le Qiu, Hui Fang, Munir M. Zaman, Edward Vitkin, Ionita C. Ghiran, Saira Salahuddin,Mark Modell, Charlotte Andersson, Lauren M. Kimerer, Patsy B. Cipolloni, Kee-Hak Lim, Steven D. Freedman,
Irving Bigio, Benjamin P. Sachs, Eugene B. Hanlon, and Lev T. Perelman
PNAS October 30, 2007 vol. 104 no. 44 17255–17260
J. Phys.: Condens. Matter 19 (2007) 113102
Schematic of an acousto-optic deflector. The deflector consists of a crystal of a material such as Tellurium oxide (blue) to which is bonded a transducer, consisting of a layer of piezoelectric material (yellow) across which an RF voltage is applied. Sound waves propagate across the crystal and (ideally) are absorbed on the other side (brown). A laser beam (orange) incident at the Bragg angle (θ) is efficiently diffracted by the grating formed by the sound waves. The first order diffracted spot contains about 80% of the incident light and is deflected by an angle φ. Some of the light remains undiffracted and some is found in higher diffracted orders. The value of φ can be changed by changing the frequency of the sound wave; the intensity of the first order spot by changing the amplitude.
OptiScan FIVE 1 confocal microscope scanhead. Image courtesy of Optiscan Pty. Ltd. (Victoria, Australia).
Early osteoarthritic changes and chondrocyte transplant success in sheep. (A) Noninvasive in vivo imaging of chondrocytes and lacunae in articular cartilage. (B) In a sheep model of cartilage damage, dramatic changes are observed at the site of tissue injury. (C) Following matrix-induced autologous chondrocyte implantation (MACI), there is a dramatic increase in the number of chondrocytes. (D) Assessement of the efficacy of cartilage repair techniques such as MACI. Image courtesy of Drs. Chris Jones and Brett Kirk, University of Western Australia, Perth, Western Australia