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Tomasz Stadejek

Impact of PRRSV diversity on diagnostic activities. Tomasz Stadejek. Russia Belarus Lithuania Western Europe, Asia, North America. Type 1 PRRSV ( European ) belongs to several distinct subtypes. ORF5 identity down to 69.5% ( Clustal V). ORF7 identity down to 75.4% ( Clustal V).

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Tomasz Stadejek

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  1. Impact of PRRSV diversity on diagnostic activities Tomasz Stadejek

  2. Russia Belarus Lithuania Western Europe, Asia, North America Type 1 PRRSV (European) belongs to severaldistinctsubtypes ORF5 identity down to 69.5% (Clustal V) ORF7 identity down to 75.4% (Clustal V)

  3. Type 1, subtype 2: 378, 375 ORF7 polymorphism Type 1, atypical?: 393 Type 1, subtype 4: 375 Type 1, subtype 1: 387, 390, 399 Type 1, subtype 3: 375, 387 Type 1, atypical?: 378 Type 2: 372, 375 +----- ----+--------- +------- --+--------- +---------+---------+------- 30 40 130 140 360 370 380 +----- ----+--------- +------- --+--------- +---------+---------+------- subtype 1 Lelystad 1991 NL-387 AAAGAA---AAGTACAGCTCCGAGGACAGGC---CAAAAAGAAAAA TACATCCGCCAGTCAGGGTGCAAGTTAA subtype 1_776 RO-390 variant AAAAAACAAAAACACAGCTCCGA GGACAGGC---AAAAAGGAAAAA TGCATCCGCCAGTCAGGGTGCAAGTTAA subtype 1_28M_2009 SK-399 variantGAAGAA---AAGTACAGCTCCAAGGACAGGC---CAGAAAGAAAAA CACATCCGCGAGTCAGGGTGCAAGTCAATTTGGTAGTTAG subtype 2 IL-1999 RU-378 AAATAA---AAATACAGCTCCAA GGACGGGCTAATAAAAAGAGGAA CGCATCTGCCAGTTAA subtype 2 Ili 2_2009 RU-375 variantAAACAA---AAATACAGCTCCAAGGACGGGT---TAAAAAGAGGAA CGCATCCGCCAGTTAG subtype 3 Soz 2004 BY-375 AAAGAA---AAATACAGCTCCAAGGACAGGA---AAAAAAAAAAAG CACGCCCGTTAGTTAA subtype 3 Soz_2009_BY-387 variantAAAGAA---AAGTACAGCTCCAAGGACAGGA---AAAAAGGAAAAG CACGCCCACTAGTCAAGGCGCAAGTTAA subtype 4_Okt BY-375 AAAGAA---AAATGTAGCTCCAAGGACAGGC---CAAAAGGAAAAA TACATCCAATAGTTAG atyp Bor-54 BY-393 AAATAA---AAACACAGCTCTAA GGACAGGCTCACAAAAGGAAAAA CGCACCCAATGATTTGGGCGCAACTCAATAG atyp VL RU-378 ACAAATCAAAAACACAACTCCAA GGACAGGC---CAAAAAGAAAAG CGCGCCTGCTAGTTAA

  4. LV 40 50 Lelystad 387sub1 QPRGGQA-KKKKP Czechy_47 387sub1_070118 QPRGGQA-RKKRP AS_34M 387sub1EE QPRGGQA-KKKKP Eigir LT 2006 378sub2 QPRGGQASKRKKP Ili_6_100210 378sub2 QPRGGRVNKRKKP Ili_9_100210 375sub2 QPRGGRV-KKRKP Zad_1_041201 375sub3 QPRGGQA-KKKKP Obu_1_050310 375sub3 QPRGGQA-KKKRP Yuz_34_050310 375sub3 QPRGGQA-KKKRP Soz_091120 387sub3STOPCOD QPRRGQE-KRKRP Soz 2A_85_85_060215 375sub3 QPRRGQG-IKKKP Okt-46 375atyp QPKGGQA-KRKKP Bor_59 393atyp_091209 QPRGGQANKRKKP 2 1 3

  5. Courtesy of AnetteBotner

  6. Vietnamese PHFD PRRSV isolate Belarusiansubtype 3 PRRSV isolate

  7. NADIR experiment DTU Vet Lindholm, Denmark • To assessvirulence of east Europeanvariants of type 1 PRRSV • PAM isolation • Pigsfreefrom 29 knownpigpathogens, intranasallyinoculated • 4 groups • 7 pigsinoculatedwithDanishisolate18794subtype 1 • 7 pigsinoculatedwithRussianisolateIli subtype 2 • 7 pigsinoculatedwithBelarusianatypicalisolateBor • 7 pigsshaminoculated • Monitored for 24 dpi 9 VET LIndholm

  8. Development of body temperature in pigs infected with type 1 PRRSVstrains 18794 (subtype 1) Ili (subtype 2) Bor (atypical) controls Courtesy of Jens Nielsen VET LIndholm

  9. Canwildboarstransmit East Europeanstrainswestward? Stankevicius et al., Barcelona 2011 ESVV 2012, Sep 5, Madrid

  10. NextGen PRRS (Tetracore) Adiavet PRRS EU/NA (Adiagene) TaqVet PRRS Triplex (LSI) Toplak et al.: J Vir Meth

  11. ESVV 2012, Sep 5, Madrid

  12. Genotype 2 PRRSV in Europe Poland 98.7% Spain 97.7% Poland 98% Germany 96% Hungary 95.7% Poland 96.2% Poland 93.7% Germany 91.2% Hungary 87.7% % identity to Ingelvac PRRS MLV Clustal W

  13. Discriminatory ELISA in a herdco-infectedwithtwogenotypes

  14. Jens Nielsen AnetteBotner Martin Oleksiewicz TorbenStorgaard ArunasStankevicius Denis Potapchuk AlexeiScherbakov Ewelina Skrzypiec Katarzyna Szymanek Michal Fabisiak RafalSapierzynski Katarzyna Podgorska Artur Jablonski Marian Porowski Iwona Stankiewicz Lars E. Larsen LiseKvisgaard Gyula Balka Daniel Cadar GabiSchageman Oliver Duran Fred Leung ManreetBrar Jose Angulo MikeRoof Eric Nelson Rolf Rauh Thankyou! ESVV 2012, Sep 5, Madrid

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