Comparison of CD34 (+) Stem Cell Enumeration by Flowcytometry in Cord Blood, Bone Marrow & Peripheral Blood.
NAME: LAXMI RATHOR
ROLL NO: 10112017
FROM: FORTIS HOSPITAL NOIDA
GUIDE: Dr. NEENA VERMA
RESULT AND DISSCUSSION
Stem cells are a class of undifferentiated cells that are able to differentiate into specialized cell types .Commonly stem cells come from two main sources:
1. Embryos formed during the blastocyst phase of embryological development (embryonic stem cells)
2.Adult tissue (adult stem cells)Both types are generally characterized by their potency or to differentiate into different cell types (such as skin , muscle , bone (etc.)
What makes stem cells so important?
Stem cells make the 3 main types of blood cells: -
Red blood cells:-Red blood cells (RBCs) carry oxygen from the lungs to all of the body,and then bring carbon dioxide back from the lungs to be exhaled,A blood test called the hematocrit show how much of your blood is made up of RBCs.
Platelets (thrombocyte):-Platelets are pieces of cell that seal damaged blood vessels and help blood to clot ,both of which are important in stopping bleeding.
The equipment used for CD34 enumeration was :-
Fig.BD Facscalibur Flowcytometry
Result & Disscussion
Types of samples:
Age Distribution of cases:-The age ranged from 0 years to 65 years.
TheSex Distribution of cases:-
Result of Cord Blood Samples:-
Result of PBSCH Samples:-
Result of Bone Marrow Samples:-
The analysis of Abs stem cells and % WBC of my showed the following samples:
Figure 2.Show the absolute stem cell counts
Figure 3.Show the % of WBC