1 / 54

Biostatistics in Practice

Biostatistics in Practice. Session 1: Quantitative and Inferential Issues. Youngju Pak Biostatistician Peter D. Christenson http://research.LABioMed.org/Biostat. Class Note. We will typically have many more slides than are covered in class. Session 1 Objectives.

svein
Download Presentation

Biostatistics in Practice

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Biostatistics in Practice Session 1: Quantitative and Inferential Issues Youngju Pak Biostatistician Peter D. Christenson http://research.LABioMed.org/Biostat

  2. Class Note We will typically have many more slides than are covered in class.

  3. Session 1 Objectives General quantitative needs in biological research Statistical software Protocol examples, with statistical sections Overview of statistical issues using a published paper

  4. Session 1 Objectives General quantitative needs in biological research Statistical software Protocol examples, with statistical sections Overview of statistical issues using a published paper

  5. General Quantitative Needs • Descriptive: Appropriate summarization to meet scientific questions: e.g., • changes, or % changes, or reaching threshold? • mean, or minimum, or range of response? • average time to death, or chances of dying by a fixed time?

  6. General Quantitative Needs, Cont’d • Inferential: Could results be spurious, a fluke, due to “natural” variations or chance? • Sensitivity/Power: How many subjects are needed? • Validity: Issues such as bias and valid inference are general scientific ones, but can be addressed statistically.

  7. Session 1 Objectives General quantitative needs in biological research Statistical software Protocol examples, with statistical sections Overview of statistical issues using a published paper

  8. Professional Statistics Software Package Output Stored data; access-ible. Enter code; syntax.

  9. Typical Statistics Software Package Select Methods from Menus www.ncss.com www.minitab.com Output after menu selection Data in spreadsheet

  10. Microsoft Excel for Statistics • Primarily for descriptive statistics. • Limited output. • No analyses for %s.

  11. www.statcrunch.com Almost Free On-Line Statistics Software • Run from browser, not local. • Can store data, results on statcrunch server. • $5/ 6 months usage.

  12. www.systat.com Free Statistics Software: Mystat

  13. www.stat.uiowa.edu/~rlenth/Power Free Study Size Software

  14. Session 1 Objectives General quantitative needs in biological research Statistical software Protocol examples, with statistical sections Overview of statistical issues using a published paper

  15. Typical Statistics Section of Protocol • Overview of study design and goals • Randomization/treatment assignment • Study size • Missing data / subject withdrawal or incompletion • Definitions / outcomes • Analysis populations • Data analysis methods • Interim analyses

  16. Public Protocol Registration www.clincialtrials.gov www.controlled-trials.com Attempt to allow the public to be aware of studies that may be negative. Many journals now require registration in order to consider future publication.

  17. Public Protocol Registration

  18. Example of Protocol--- Displayed in Class ---

  19. Session 1 Objectives General quantitative needs in biological research Statistical software Protocol examples, with statistical sections Overview of statistical issues

  20. Statistical Issues Subject selection Randomization Efficiency from study design Summarizing study results Making comparisons Study size Attributability of results Efficacy vs. effectiveness Exploring vs. proving

  21. Paper with Common Statistical Issues Case Study:

  22. McCann, et al., Lancet 2007 Nov 3;370(9598):1560-7 • Food additives and hyperactive behaviour in 3-year-old and 8/9-year-old children in the community: a randomised, double-blinded, placebo-controlled trial. • Target population: 3-4, 8-9 years old children • Study design: randomized, double-blinded, controlled, crossover trial • Sample size: 153 (3 years), 144(8-9 years) in Southampton UK • Objective: test whether intake of artificial food color and additive (AFCA) affects childhood behavior • Sampling: Stratified sampling based on SES • Baseline measure: 24h recall by the parent of the child’s pretrial diet • Group: three groups (mix A, mix B, placebo) • Outcomes: ADHD rating scale IV by teachers, WWP hyperactivity score by parents, classroom observation code, Conners continuous performance test II (CPTII)  GHA score

  23. Statistical Issues Subject selection Randomization Efficiency from study design Summarizing study results Making comparisons Study size Attributability of results Efficacy vs. effectiveness Exploring vs. proving

  24. Selecting Study Subjects

  25. Representative or Random Samples How were the children to be studied selected (second column on the first page)? The authors purposely selected "representative" social classes. Is this better than a "randomly" chosen sample that ignores social class? Often hear: Non-random = Non-scientific.

  26. Case Study: Participant Selection No mention of random samples.

  27. Case Study: Participant Selection It may be that only a few schools are needed to get sufficient individuals. If, among all possible schools, there are few that are lower SES, none of these schools may be chosen. So, a random sample of schools is chosen from the lower SES schools, and another random sample from the higher SES schools.

  28. Selection by Over-Sampling It is not necessary that the % lower SES in the study is the same as in the population. There may still be too few subjects in a rare subgroup to get reliable data. Can “over-sample” a rare subgroup, and then weight overall results by proportions of subgroups in the population. The CDC NHANES studies do this.

  29. Random Samples vs. Randomization We have been discussing the selection of subjects to study, often a random sample. An observational study would, well, just observe them. An interventional study assigns each subject to one or more treatments in order to compare treatments. Randomization refers to making these assignments in a random way.

  30. Why Randomize? Plant breeding example: Compare yields of varieties A and B, planting each to 18 plots. Which design is better? Systematic Randomized

  31. Why Randomize? So that groups will be similar except for the intervention. So that, when enrolling, we will not unconsciously choose an “appropriate” treatment for a particular subject. Minimizes the chances of introducing bias when attempting to systematically remove it, as in plant yield example.

  32. Statistical Issues Subject selection Randomization Efficiency from study design Summarizing study results Making comparisons Study size Attributability of results Efficacy vs. effectiveness Exploring vs. proving

  33. Basic Study Designs • Prospective (longitudinal) • Retrospective(Case-Control) • Cross sectional • Randomized-Control

  34. Case Study: Crossover Design Each child is studied on 3 occasions under different diets. Is this better than three separate groups of children? Why, intuitively? How could you scientifically prove your intuition?

  35. Blocked vs. Unblocked Studies AKA matched vs. unmatched. AKA paired vs. unpaired. Block = Pair = Set receiving all treatments. Set could be an individual at multiple times (pre and post), or left and right arms for sunscreen comparison; twins or family; centers in multi-center study, etc. Block ↔ Homogeneous. Blocking is efficient because treatment differences are usually more consistent among subjects than each separate treatment is.

  36. Potential Efficiency Due to Pairing Unpaired Paired A and B Separate Groups A and B in a Paired Set . . . … . . . . . .. . . . . . . .. . . . . . . . . . .. . . . . . . . . … . .. .. .. . . . . . . . . … . .. .. .. Δ 3 3 …….… …. …….… …. 3 A B A B Δ=B-A

  37. Statistical Issues Subject selection Randomization Efficiency from study design Summarizing study results Making comparisons Study size Attributability of results Efficacy vs. effectiveness Exploring vs. proving

  38. Outcome Measures Generally, how were the outcome measures defined (third page)? They are more complicated here than for most studies. What are the units (e.g., kg, mmol, $, years)? Outcome measures are specific and pre-defined. Aims and goals may be more general.

  39. Summarization / Data Reduction How are the outcome measures summarized? e.g., Table 2:

  40. Case Study: Statistical Comparisons How might you intuitively decide from the summarized results whether the additives have an effect? Different Enough? Clinically?Statistically?

  41. Statistical Comparisons: Figure 3

  42. Statistical Comparisons and Tests of Hypotheses Engineering analogy: Signal and Noise Signal = Diet effect Noise = Degree of precision Statistical Tests: Effect is probably real if signal-to-noise ratio Signal/Noise is large enough. Importance of reducing “noise”, which incorporates subject variability and N.

  43. Back to Efficiency of Design Unpaired Paired A and B Separate Groups A and B in a Paired Set . . . … . . . . . .. . . . . . . .. . . . . . . . . . .. . . . . . . . . … . .. .. .. . . . . . . . . … . .. .. .. Δ Noise 3 3 …….… …. …….… …. Noise 3 A B A B Δ=B-A Signal = 3

  44. Statistical Issues Subject selection Randomization Efficiency from study design Summarizing study results Making comparisons Study size Attributability of results Efficacy vs. effectiveness Exploring vs. proving

  45. Number of Subjects The authors say, in the second column on the fourth page: Intuitively, what should go into selecting the study size? We will make this intuition rigorous in Session 4.

  46. Statistical Issues Subject selection Randomization Efficiency from study design Summarizing study results Making comparisons Study size Attributability of results Efficacy vs. effectiveness Exploring vs. proving

  47. Other Effects, Potential Biases The top of the second column on the fourth page mentions other effects on diet: The issue here is: Could apparent diet differences (e.g., -0.26 B vs. -0.44 Placebo) be attributable to something else?

  48. Statistical Issues Subject selection Randomization Efficiency from study design Summarizing study results Making comparisons Study size Attributability of results Efficacy vs. effectiveness Exploring vs. proving

  49. Non-Completing or Non-Adhering Subjects What is the most relevant group of studied subjects: all randomized, mostly adherent, fully adherent? Study Goal: Scientific effect? Societal impact?

  50. Statistical Issues Subject selection Randomization Efficiency from study design Summarizing study results Making comparisons Study size Attributability of results Efficacy vs. effectiveness Exploring vs. proving

More Related