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Eau d’coli

Eau d’coli. N Kuldell for 20.020 Spring 2009. WGD. and. BGD. WGD: Wintergreen Generating Device. BGD: Banana Generating Device. System level description: initial planning. Black box functions. Input e.g. precursor. Output e.g. smell. System level description: scent production. WGD.

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Eau d’coli

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  1. Eau d’coli N Kuldell for 20.020 Spring 2009

  2. WGD and BGD WGD: Wintergreen Generating Device BGD: Banana Generating Device System level description: initial planning Black box functions Input e.g. precursor Output e.g. smell

  3. System level description: scent production WGD Black box functions Input e.g. precursor Output e.g. smell BGD WGD: Wintergreen Generating Device BGD: Banana Generating Device

  4. System level description: scent production WGD Black box functions Input e.g. precursor Output e.g. smell BGD WGD: Wintergreen Generating Device BGD: Banana Generating Device

  5. System level description: precursor production WGD Black box functions Input e.g. cell growth Output e.g. smell BGD saGD and iaGD WGD: Wintergreen Generating Device BGD: Banana Generating Device saGD: salicylic acid Gen Dev iaGD: isoamyl alcohol Gen Dev

  6. System level description: precursor production Input e.g. cell growth saGD WGD Black box functions Output e.g. smell iaGD BGD WGD: Wintergreen Generating Device BGD: Banana Generating Device saGD: salicylic acid Gen Dev iaGD: isoamyl alcohol Gen Dev

  7. cell density time System level description: growth regulation Input e.g. cell growth saGD WGD Output e.g. smell iaGD BGD “stationary” “log” “lag”

  8. “stationary” cell density “log” “lag” time System level description: growth regulation Input e.g. log growth saGD WGD ON OFF iaGD BGD wintergreen smell

  9. “stationary” cell density “log” “lag” time System level description: growth regulation Input e.g. stationary growth saGD WGD OFF ON iaGD BGD banana smell

  10. System level description: growth regulation Input e.g. stationary growth saGD WGD OFF ON iaGD BGD OsmY is a promoter that is only active during stationary phase

  11. System level description: growth regulation Input e.g. stationary growth saGD WGD OFF ON iaGD BGD Stationary phase Log phase OsmY 1 0 OsmY+ inverter 0 1

  12. System level description: growth regulation Input e.g. stationary growth saGD WGD OFF ON iaGD BGD

  13. System level description: Chassis modification Input e.g. stationary growth saGD WGD OFF ON iaGD BGD eliminate natural indole production

  14. E. coli + precursor (SA) + WGD E. coli methyl salicylate MW = 152 no methyl salicylate [no peak at 9.1 minutes] System level test: gas chromatographs

  15. System level test: uninformed experimental subjects??

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