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Fig S1. b. a. Cortex. Cortex. 20. 20. *. 16. 16. *. 12. 12. MAGL (fmol/mg/min). FAAH (fmol/mg/min). *. 8. 8. *. 0 mg/kg. *. 4. 4. 0 mg/kg. 1 mg/kg. 1 mg/kg. 0. 0. 0 1h 1d 2d 3d 10d. 0 1h 1d 2d 3d 10d. Time relative to injection.

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Fig s1

Fig S1

b

a

Cortex

Cortex

20

20

*

16

16

*

12

12

MAGL (fmol/mg/min)

FAAH (fmol/mg/min)

*

8

8

*

0 mg/kg

*

4

4

0 mg/kg

1 mg/kg

1 mg/kg

0

0

0 1h 1d 2d 3d 10d

0 1h 1d 2d 3d 10d

Time relative to injection

Time relative to injection

d

c

Cerebellum

Cerebellum

120

120

100

100

80

80

MAGL (% 0 timepoint)

FAAH (% 0 timepoint)

60

60

40

40

0 mg/kg

0 mg/kg

20

20

1 mg/kg

1 mg/kg

0

0

0 1h 1d 2d 3d 10d

0 1h 1d 2d 3d 10d

Time relative to injection

Time relative to injection

f

e

Cerebellum

Cerebellum

20

20

16

16

12

12

MAGL (fmol/mg/min)

FAAH (fmol/mg/min)

8

8

0 mg/kg

0 mg/kg

4

4

1 mg/kg

1 mg/kg

0

0

0 1h 1d 2d 3d 10d

0 1h 1d 2d 3d 10d

Time relative to injection

Time relative to injection


Fig s1

Fig S2

a

b

VEH

0.5 mg/kg

VEH

100

100

0.25 mg/kg

1.0 mg/kg

AM

80

80

60

60

Percent freezing

Percent freezing

40

40

20

20

0

0

Baseline

Last

trial-block

Baseline

Last

trial-block

First

trial-block

First

trial-block

c

d

VEH

100

100

AM

80

80

60

60

Percent freezing

Percent freezing

40

40

20

20

0

0

AM

VEH

Baseline

Last

trial-block

First

trial-block


Fig s1

Fig S3

b

a

*

*

VEH

400

URB

400

AM

300

*

300

*

AUC (mg h/dl)

Glucose (mg/dl)

200

200

100

100

0

0

URB

AM

VEH

0

15

30

45

60

90

120

150

180

Time (min)


Fig s1

Fig S4

a

b

Amygdala

Cortex

Striatum

15

15

12

12

9

9

2-arachidonoylglycerol

(pmol/mg protein)

2-arachidonoylglycerol

(pmol/mg protein)

6

6

3

3

0

0

VEH

AM

VEH

VEH

AM

AM

CS

CS

CS

CS

CS

CS

CS-US

CS-US

CS-US

CS-US

CS-US

CS-US


Fig s1

Fig S5

a

b

*

AM3506

110

120

100

100

90

80

fEPSP amplitude

(% baseline)

fEPSP amplitude

(% baseline)

80

60

70

40

60

20

50

0

0 20 30 40 50 60 70 80 90 100

Baseline

Last 5 min

Time (min)

c

d

LFS

120

120

100

100

80

80

fEPSP amplitude

(% baseline)

60

fEPSP amplitude

(% baseline)

60

40

40

VEH

20

20

AM

0

0

-10 0 10 20 30 40 50 60 70 80

Base

Last 5

Base

Last 5

Time relative to LFS (min)

VEH

AM


Fig s1

Fig S6

a

VEH

100

AM

AM+SR

SR

80

60

Percent freezing

40

20

0

Baseline

Last

trial-block

First

trial-block

b

VEH

100

AM

AM+SRBLA

80

60

Percent freezing

40

20

0

Baseline

Last

trial-block

First

trial-block

c

VEH

100

AMBLA

80

60

Percent freezing

40

20

0

Baseline

Last

trial-block

First

trial-block


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