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Water structures carrying DNA information, Application to HIV/AIDS and Autism. L.Montagnier, Borovets, October 23, 2013. The Evolution of Medicine Toward 4 P Qualities P1 Preventive P2 Predictive P3 Personalized P4 Participative. Emergence or reemergence of new epidemics due to :

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Presentation Transcript
slide1

Water structures carrying DNA information, Application to HIV/AIDS and Autism

L.Montagnier, Borovets, October 23, 2013

slide2
The Evolution of Medicine

Toward 4 P Qualities

P1 Preventive

P2 Predictive

P3 Personalized

P4 Participative

slide3

Emergence or reemergence

  • of new epidemics due to :
  • Globalization of exchanges and travels
  • Demography: concentration in large cities
  • Nutrition (pesticides, water)
  • Environmental factors
  • Climate changes, electromagnetic radiations
  • Contacts with wild and farm animals
  • Decline of immune defenses
slide4

- Cancers- Cardiovascular- Neurodegenerative- Arthritic- Autoimmune- Multifactorial, but in common: oxidative stress infectious agents (?)

The most important burdenChronic Diseases

various environmental factors effects accumulate
Radiations (, X, UV, visible, hertzian)

Air chemical pollution

Inadequate Food

Excessive physical exercize

tobacco smoke

alcohol

ischemia

Parasitic infections

Bacterial infections

Viral infections

Various environmental factors effects accumulate

+

+

+

+

+

+

+

+

+

= DISEASE

slide6
Lipid hyperoxydation (plasma, cell membranes)

Protein Oxydation --> destruction, agregates

DNA oxydation --> Mutations, Chromosomal Breakage

Pathological effects of Oxydative Stress

slide7

Oxidative stress

Weakens the immune system

Activates transcription factors

(NF-kappa B)

Activates genes involved

in cell division, inflammatory cytokines,

lymphocytes activation

Immune dysfunction, apoptosis

(TH1 TH2)

slide8

The Human Genome

Genes (+ introns +promoters) 3%

Retroelements: retroviruses 8%

retrotransposons 13%

LTR 23%

Total 47%

Repeats 50%

LINE 22%

SINE 13%

Unknown ?

mucosa skin

MucosaSkin

Our microbial ecosystem :

Constant exposure to microbial agents and immune protection

slide10

Extreme genetic plasticity

  • of microorganisms:
  • virus : HIV, Influenza
  • Bacteria (plasmids)
  • Parasites (retrotransposons)
  • against reaction of the immune system
biofilms mobile antibiotic resistant genes nanoforms nanostructures endosymbiontes
- Biofilms- Mobile antibiotic resistant genes- Nanoforms- Nanostructures- Endosymbiontes

How bacteria have learned how to persist despite the immune system

slide12

Parasite Bacterium Virus

(Worm) intracellular

Filarial nematode Wolbachia

slide13

Persistent cold infections

  • Antibiotics affect them only when they go out of sanctuaries
  • Inability of the immune system to eradicate them
  • (tolerance, oxidative stress)
  • Non-multiplicative forms of « classical » bacteria
  • intracellular bacteria
  • Sanctuaries
  • (bone marrow, joints, intestine, brain, ….)
  • Vectors (Parasites)
  • Remote effects (toxin, nanostructures)
slide15

Two technologies for detecting bacteria and viral DNA’s

  • A new technology based on the production of electromagnetic waves
  • The Polymerase Chain Reaction (PCR) using the 16S ribosomal DNA variability
slide16

Classical model of PCR

oligo

Taq

polymerase

oligo

resonance emission of low frequency electromagnetic waves by high water dilutions of dna
Resonance emission of low frequency electromagnetic waves by high water dilutions of DNA.

A newly discovered property of DNA :

molecular recognition without close contact by waves and resonance
Molecular recognition

without close contact

(by waves and resonance)

slide19

Capture of the signals

Signal Analysis

software

Sample

X 500

Sensor coil

Computer

Amplifier

slide20

Detection of Ultra Low Frequencies Waves (ULF 500-2000 hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

FACTS

slide21

7-100 Hz

Filtration

0.1

0.02

2ng/1ml 10-2 10-3 10-4 10-5 10-6 10-7 10-8 10-9 10-10 10-12 10-13 10-14 10-15 10-16

1000 -

3000 Hz

slide22

Noise

Amplitude

(+)

Time (sec)

slide24

Spectral Frequency Analysis

Fourier

Tranformation

  • A positive signal is defined by:
    • amplitude increase
    • Shift to higher frequencies (500-2000 Hertz)
slide25

Noise

(+)

Time (sec)

Frequency (1-20000 Hertz)

slide26

Suspensions of pure culture ofbacteria with pathogenic potentialare producing electromagnetic signals (EMS) in the range of dilution usually 10-810-13, sometime (E.coli) up to 10-18

They are:E.coli, Streptococcus, Salmonella, Staphylococcus, B.subtilis, Clostridium, Pseudomonas, etc.

slide31

Micro-organisms involved

in EMS induction

1.DNA from main pathogenic bacteria

Streptococcus

Staphylococcus

Pseudomonas

Mycoplasma pirum

Salmonella

Clostridium

Proteus mirabilis

B. Subtilis

Borrelia burgdorferi

Sutterella

- From viruses HIV1

Influenza group A

HBV

HCV

- Genes involved M.pirum adhesin

HIV genes

slide32

10-2

Water excitated state

crystal like gel

no EMS

DNA

Interpretation

10-9

Free polymer

self maintained

by EMS

slide33

I – DNA’s emit EMS

II – EMS are produced by water nanostructures (naneons)

III – EMS are producing naneons

IV – Naneons and EMS carry specific DNA information

slide34

EMS are produced by water

  • nanostructures (naneons)
  • Evidence :
  • from filtration
  • Size : between 20 and 100 nM for bacterial sequences
  • Smaller that 20µM for viral sequences
  • from biophysical studies
  • indicating spectrometral changes in the dilutions
  • producing EMS
slide35

Naneons and EMS carry

specific DNA information

Natural and digital transmission

slide36

Classical model of PCR

oligo

Taq

polymerase

oligo

slide38

Water Naneons

DNA

EMS EMS

Water Naneons

PCR

DNA

7 Hz

slide39

Generator 7Hz

DNA

-6

-6

water

Tube 1

Tube 2

µmetal

-2 -3 -4 -5 -6

18hrs

water

+

+

+

EMS

slide40

D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM

Transmission in water of D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM

DW: Distilled Water / FD2: Dilution 10-2 after filtration 450and 20 nM

slide41

Water-mediated photonic transmission of DNA

Water Naneons

DNA

Computer

Digitized

EMS

Receiver

Computer

PCR

EMS

Analog

DNA

Water Naneons

slide42

Reproduction of DNA transduction

in other laboratories

File EMS of 194 bp DNA from HIV1 LTR

Sent to Benevento University,

Molecular Biology Laboratory

DNA reproduced and sequenced

100 % identical to original

File EMS of 499 bp DNA from Borrelia burgdorferi

Sent to Laboratory of Chronix Biomedicals

University of Gottingen

slide43

Reproduction of DNA transduction in other laboratories

File EMS of 194 bp DNA from HIV1 LTR

Sent to Benevento University,

Molecular Biology Laboratory

DNA reproduced and sequenced

100 % identical to original

File EMS of 499 bp DNA from Borrelia burgdorferi

Sent to Laboratory of Chronix Biomedicals

University of Gottingen

slide44

Water-mediated photonic transmission of DNA

Gel electrophoresis of the PCR DNA product (Borrelia Burgdorferi)

E.Schutz et al. Goettingen, 2011

slide45

ELF

DNA

DNA

DNA

WATER

DNA

(plasma)

DNA

How pathogenic information can persist, and escape immune defence and treatment.

slide47

The dual origin of EMS in HIV/AIDS

EMS plasma RBC

HIV < 20 nM ++ +/-

Agent X< 100 nM - ++

slide50

Evidence that the agent X is a bacterium

  • Presence of a sequence close to 16 S ribosomal DNA
  • of Rickettsiales
  • Sensitive to antibiotics ( in vitro and in vivo)
  • Growth in cell line
  • Electron microscopy
  • Associated with Red Blood Cells
slide52

Human sequences associated with the agent X237 bp (human chromosome 1)213 bp (human chromosome 7)and more…

slide54

Strong correlation with HIV infection

  • Contains human DNA sequences emitting EMS
  • only in pathogenic HIV infection
  • Sequence 213 bp from Chromosome 7
  •  EMS in HIV + patients
  •  no EMS in HIV - patients
slide55

DNA

PCR

EMS

+

+

(20 nM)

HIV plasma

RBC

(LTR 195 bp)

(all HIV)

+

+

(100 nM)

(213/237 bp)

Risk factor 2

RBC

(HIV+)

( all HIV+, a few HIV-)

+

-

Risk factor 1

RBC

(HIV- & HIV+)

( all HIV+, many HIV-)

(100 nM)

RISK FACTORS OF HIV INFECTION

slide56

CONCLUSION 1

« A new intracellular bacterium has been found in human red blood cells. A variant bearing extra DNA sequences is present in all HIV infected patients and also in some healthy HIV negative African individuals”.

slide57

CONCLUSION 2

  • Origin of EMS :
  • not necessarily depending on DNA sequence
  • depending on transmissible modification of DNA
  • (free radicals of water ?)
  • - Association with pathogenicity
slide58

AUTISM :

THE GUT, BLOOD and BRAIN

MICROBIAL CONNECTION

slide68

Correlation between EMS, antibiotic treatment

and clinical signs in an autistic child

% EMS/noise

40 -

AZITHROMYCIN

CEFUROXIM

30 -

Accept various food

More present with

Family members

Starts speaking

20 -

10 -

0 -

days

27

50

100

133

slide69

Environmental factors

genetic susceptibility

Oxidative stress

immunosuppression

Bacterial agents

Oxidative stress

Somatic mutations

« prion » effect

Less and less reversible

Reversible

slide72

In press

Electromagnetic detection of HIV DNA in the blood of AIDS patients

treated by antiretroviral therapy.

Luc MONTAGNIER†×^, Jamal AISSA†, Claude LAVALLEE×,

Mireille MBAMY⁰, Joseph VARON#, and Henri CHENAL⁰

× World Foundation for AIDS research and Prevention (UNESCO), 1 rue Miollis, 75015, Paris, France

† Nanectis Biotechnologies, France

⁰ CIRBA Centre Intégré de Recherches Biocliniques d’Abidjan, Ivory Coast

# The University of Texas Health Science Center, Houston, USA.

Abstract : Electromagnetic signals of low frequency have been shown to be durably produced in aqueous dilutions of the Human Imunodeficiency Virus DNA. In vivo, HIV DNA signals are detected only in patients previously treated by antiretroviral therapy and having no detectable viral RNA copies in their blood. We suggest that the treatment of AIDS patients pushes the virus towards a new mode of replication implying only DNA, thus forming a reservoir insensitive to retroviral inhibitors. Implications for new approaches aimed at eradicating HIV infection are discussed.

Key words: DNA, Electromagnetic signals, bacteria

slide74

World Foundation of AIDS

Research and Prevention

R. Olivier, Cl. Lavallee,

H.Chenal, M. Mbamy,

Nanectis Biotechnologies SA

J.Aissa, Cl.Lavallee, R.Olivier

Goettingen University and Chronix Biomedicals

E. Schutz, H.Urnovitz

University of Washington, Seattle

Gerald H. Pollack

slide75

“Absence of evidence

is not

evidence of absence”

Carl Sagan

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