Water structures carrying DNA information, Application to HIV/AIDS and Autism
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Water structures carrying DNA information, Application to HIV/AIDS and Autism. L.Montagnier, Borovets, October 23, 2013. The Evolution of Medicine Toward 4 P Qualities P1 Preventive P2 Predictive P3 Personalized P4 Participative. Emergence or reemergence of new epidemics due to :

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Water structures carrying DNA information, Application to HIV/AIDS and Autism

L.Montagnier, Borovets, October 23, 2013


The Evolution of Medicine HIV/AIDS and Autism

Toward 4 P Qualities

P1 Preventive

P2 Predictive

P3 Personalized

P4 Participative


  • Emergence or reemergence HIV/AIDS and Autism

  • of new epidemics due to :

  • Globalization of exchanges and travels

  • Demography: concentration in large cities

  • Nutrition (pesticides, water)

  • Environmental factors

  • Climate changes, electromagnetic radiations

  • Contacts with wild and farm animals

  • Decline of immune defenses


- HIV/AIDS and AutismCancers- Cardiovascular- Neurodegenerative- Arthritic- Autoimmune- Multifactorial, but in common: oxidative stress infectious agents (?)

The most important burdenChronic Diseases


Various environmental factors effects accumulate

Radiations ( HIV/AIDS and Autism, X, UV, visible, hertzian)

Air chemical pollution

Inadequate Food

Excessive physical exercize

tobacco smoke

alcohol

ischemia

Parasitic infections

Bacterial infections

Viral infections

Various environmental factors effects accumulate

+

+

+

+

+

+

+

+

+

= DISEASE


Lipid hyperoxydation (plasma, cell membranes) HIV/AIDS and Autism

Protein Oxydation --> destruction, agregates

DNA oxydation --> Mutations, Chromosomal Breakage

Pathological effects of Oxydative Stress


Oxidative stress HIV/AIDS and Autism

Weakens the immune system

Activates transcription factors

(NF-kappa B)

Activates genes involved

in cell division, inflammatory cytokines,

lymphocytes activation

Immune dysfunction, apoptosis

(TH1 TH2)


The Human Genome HIV/AIDS and Autism

Genes (+ introns +promoters) 3%

Retroelements: retroviruses 8%

retrotransposons 13%

LTR 23%

Total 47%

Repeats 50%

LINE 22%

SINE 13%

Unknown ?


Mucosa skin

Mucosa HIV/AIDS and AutismSkin

Our microbial ecosystem :

Constant exposure to microbial agents and immune protection


  • Extreme genetic plasticity HIV/AIDS and Autism

  • of microorganisms:

  • virus : HIV, Influenza

  • Bacteria (plasmids)

  • Parasites (retrotransposons)

  • against reaction of the immune system


Biofilms mobile antibiotic resistant genes nanoforms nanostructures endosymbiontes
- Biofilms HIV/AIDS and Autism- Mobile antibiotic resistant genes- Nanoforms- Nanostructures- Endosymbiontes

How bacteria have learned how to persist despite the immune system


Parasite Bacterium Virus HIV/AIDS and Autism

(Worm) intracellular

Filarial nematode Wolbachia


  • Inability of the immune system to eradicate them

  • (tolerance, oxidative stress)

  • Non-multiplicative forms of « classical » bacteria

  • intracellular bacteria

  • Sanctuaries

  • (bone marrow, joints, intestine, brain, ….)

  • Vectors (Parasites)

  • Remote effects (toxin, nanostructures)


EXPLORING THE ROLE OF LATENT INFECTION IN CHRONIC DISEASES HIV/AIDS and Autism

A physical and molecular approach


Two technologies for detecting bacteria and viral DNA’s HIV/AIDS and Autism

  • A new technology based on the production of electromagnetic waves

  • The Polymerase Chain Reaction (PCR) using the 16S ribosomal DNA variability


Classical model of PCR HIV/AIDS and Autism

oligo

Taq

polymerase

oligo


Resonance emission of low frequency electromagnetic waves by high water dilutions of dna

Resonance emission of low frequency electromagnetic waves by high water dilutions of DNA.

A newly discovered property of DNA :


Molecular recognition without close contact by waves and resonance

Molecular recognition by high water dilutions of DNA.

without close contact

(by waves and resonance)


Capture of the signals by high water dilutions of DNA.

Signal Analysis

software

Sample

X 500

Sensor coil

Computer

Amplifier


Detection of Ultra Low Frequencies Waves (ULF 500-2000 hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

FACTS


7-100 Hz hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

Filtration

0.1

0.02

2ng/1ml 10-2 10-3 10-4 10-5 10-6 10-7 10-8 10-9 10-10 10-12 10-13 10-14 10-15 10-16

1000 -

3000 Hz


Noise hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

Amplitude

(+)

Time (sec)


Spectral Frequency Analysis hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

Fourier

Tranformation

  • A positive signal is defined by:

    • amplitude increase

    • Shift to higher frequencies (500-2000 Hertz)


Noise hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

(+)

Time (sec)

Frequency (1-20000 Hertz)


Suspensions of pure culture of hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.bacteria with pathogenic potentialare producing electromagnetic signals (EMS) in the range of dilution usually 10-810-13, sometime (E.coli) up to 10-18

They are:E.coli, Streptococcus, Salmonella, Staphylococcus, B.subtilis, Clostridium, Pseudomonas, etc.


Micro-organisms involved hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

in EMS induction

1.DNA from main pathogenic bacteria

Streptococcus

Staphylococcus

Pseudomonas

Mycoplasma pirum

Salmonella

Clostridium

Proteus mirabilis

B. Subtilis

Borrelia burgdorferi

Sutterella

- From viruses HIV1

Influenza group A

HBV

HCV

- Genes involved M.pirum adhesin

HIV genes


10 hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.-2

Water excitated state

crystal like gel

no EMS

DNA

Interpretation

10-9

Free polymer

self maintained

by EMS


I – DNA’s emit EMS hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

II – EMS are produced by water nanostructures (naneons)

III – EMS are producing naneons

IV – Naneons and EMS carry specific DNA information


  • EMS are produced by water hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

  • nanostructures (naneons)

  • Evidence :

  • from filtration

  • Size : between 20 and 100 nM for bacterial sequences

  • Smaller that 20µM for viral sequences

  • from biophysical studies

  • indicating spectrometral changes in the dilutions

  • producing EMS


Naneons and EMS carry hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

specific DNA information

Natural and digital transmission


Classical model of PCR hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

oligo

Taq

polymerase

oligo


PCR on water nanostructures hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

oligo

Taq

EMS

oligo


Water Naneons hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

DNA

EMS EMS

Water Naneons

PCR

DNA

7 Hz


Generator 7Hz hertz) in certain dilutions of filtrates (100nM, 20nM, 15nM) from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents.

DNA

-6

-6

water

Tube 1

Tube 2

µmetal

-2 -3 -4 -5 -6

18hrs

water

+

+

+

EMS


D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM

Transmission in water of D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM

DW: Distilled Water / FD2: Dilution 10-2 after filtration 450and 20 nM


Water-mediated photonic transmission of DNA from D-2 to D-15 after filtration 450 and 20 nM

Water Naneons

DNA

Computer

Digitized

EMS

Receiver

Computer

PCR

EMS

Analog

DNA

Water Naneons


Reproduction of DNA transduction from D-2 to D-15 after filtration 450 and 20 nM

in other laboratories

File EMS of 194 bp DNA from HIV1 LTR

Sent to Benevento University,

Molecular Biology Laboratory

DNA reproduced and sequenced

100 % identical to original

File EMS of 499 bp DNA from Borrelia burgdorferi

Sent to Laboratory of Chronix Biomedicals

University of Gottingen


Reproduction of DNA transduction in other laboratories from D-2 to D-15 after filtration 450 and 20 nM

File EMS of 194 bp DNA from HIV1 LTR

Sent to Benevento University,

Molecular Biology Laboratory

DNA reproduced and sequenced

100 % identical to original

File EMS of 499 bp DNA from Borrelia burgdorferi

Sent to Laboratory of Chronix Biomedicals

University of Gottingen


Water-mediated photonic transmission of DNA from D-2 to D-15 after filtration 450 and 20 nM

Gel electrophoresis of the PCR DNA product (Borrelia Burgdorferi)

E.Schutz et al. Goettingen, 2011


ELF from D-2 to D-15 after filtration 450 and 20 nM

DNA

DNA

DNA

WATER

DNA

(plasma)

DNA

How pathogenic information can persist, and escape immune defence and treatment.


The viral dna reservoir the higher sensitivity of african people to hiv infection

Two main problems for AIDS research from D-2 to D-15 after filtration 450 and 20 nM

in 2013

- The viral DNA reservoir- The Higher sensitivity of African people to HIV infection


The dual origin of EMS in HIV/AIDS from D-2 to D-15 after filtration 450 and 20 nM

EMS plasma RBC

HIV < 20 nM ++ +/-

Agent X< 100 nM - ++


Evidence that the agent X is a bacterium from D-2 to D-15 after filtration 450 and 20 nM

  • Presence of a sequence close to 16 S ribosomal DNA

  • of Rickettsiales

  • Sensitive to antibiotics ( in vitro and in vivo)

  • Growth in cell line

  • Electron microscopy

  • Associated with Red Blood Cells


Human sequences associated with the agent X from D-2 to D-15 after filtration 450 and 20 nM237 bp (human chromosome 1)213 bp (human chromosome 7)and more…


Strong correlation with HIV infection from D-2 to D-15 after filtration 450 and 20 nM

  • Contains human DNA sequences emitting EMS

  • only in pathogenic HIV infection

  • Sequence 213 bp from Chromosome 7

  •  EMS in HIV + patients

  •  no EMS in HIV - patients


DNA from D-2 to D-15 after filtration 450 and 20 nM

PCR

EMS

+

+

(20 nM)

HIV plasma

RBC

(LTR 195 bp)

(all HIV)

+

+

(100 nM)

(213/237 bp)

Risk factor 2

RBC

(HIV+)

( all HIV+, a few HIV-)

+

-

Risk factor 1

RBC

(HIV- & HIV+)

( all HIV+, many HIV-)

(100 nM)

RISK FACTORS OF HIV INFECTION


CONCLUSION 1 from D-2 to D-15 after filtration 450 and 20 nM

« A new intracellular bacterium has been found in human red blood cells. A variant bearing extra DNA sequences is present in all HIV infected patients and also in some healthy HIV negative African individuals”.


CONCLUSION 2 from D-2 to D-15 after filtration 450 and 20 nM

  • Origin of EMS :

  • not necessarily depending on DNA sequence

  • depending on transmissible modification of DNA

  • (free radicals of water ?)

  • - Association with pathogenicity


AUTISM from D-2 to D-15 after filtration 450 and 20 nM :

THE GUT, BLOOD and BRAIN

MICROBIAL CONNECTION


GUT BLOOD BRAIN from D-2 to D-15 after filtration 450 and 20 nM


Correlation between EMS, antibiotic treatment from D-2 to D-15 after filtration 450 and 20 nM

and clinical signs in an autistic child

% EMS/noise

40 -

AZITHROMYCIN

CEFUROXIM

30 -

Accept various food

More present with

Family members

Starts speaking

20 -

10 -

0 -

days

27

50

100

133


Environmental factors from D-2 to D-15 after filtration 450 and 20 nM

genetic susceptibility

Oxidative stress

immunosuppression

Bacterial agents

Oxidative stress

Somatic mutations

« prion » effect

Less and less reversible

Reversible


In press from D-2 to D-15 after filtration 450 and 20 nM

Electromagnetic detection of HIV DNA in the blood of AIDS patients

treated by antiretroviral therapy.

Luc MONTAGNIER†×^, Jamal AISSA†, Claude LAVALLEE×,

Mireille MBAMY⁰, Joseph VARON#, and Henri CHENAL⁰

× World Foundation for AIDS research and Prevention (UNESCO), 1 rue Miollis, 75015, Paris, France

† Nanectis Biotechnologies, France

⁰ CIRBA Centre Intégré de Recherches Biocliniques d’Abidjan, Ivory Coast

# The University of Texas Health Science Center, Houston, USA.

Abstract : Electromagnetic signals of low frequency have been shown to be durably produced in aqueous dilutions of the Human Imunodeficiency Virus DNA. In vivo, HIV DNA signals are detected only in patients previously treated by antiretroviral therapy and having no detectable viral RNA copies in their blood. We suggest that the treatment of AIDS patients pushes the virus towards a new mode of replication implying only DNA, thus forming a reservoir insensitive to retroviral inhibitors. Implications for new approaches aimed at eradicating HIV infection are discussed.

Key words: DNA, Electromagnetic signals, bacteria


Centre Intégré de Recherche Bioclinique d’Abidjan from D-2 to D-15 after filtration 450 and 20 nM


World Foundation of AIDS from D-2 to D-15 after filtration 450 and 20 nM

Research and Prevention

R. Olivier, Cl. Lavallee,

H.Chenal, M. Mbamy,

Nanectis Biotechnologies SA

J.Aissa, Cl.Lavallee, R.Olivier

Goettingen University and Chronix Biomedicals

E. Schutz, H.Urnovitz

University of Washington, Seattle

Gerald H. Pollack


from D-2 to D-15 after filtration 450 and 20 nMAbsence of evidence

is not

evidence of absence”

Carl Sagan


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