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Sponsored by the with a grant from NOAA

NE Regional Lobster Sample Repository: Data Collection and Initial Findings of the Lobster Health Coalition. Sponsored by the with a grant from NOAA. Primary Researchers. Deborah A. Bouchard – UMaine Aquatic Animal Health Lab Anne B. Lichtenwalner - UMaine Aquatic Animal Health Lab

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Sponsored by the with a grant from NOAA

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  1. NE Regional Lobster Sample Repository: Data Collection and Initial Findings of the Lobster Health Coalition Sponsored by the with a grant from NOAA

  2. Primary Researchers • Deborah A. Bouchard – UMaine Aquatic Animal Health Lab • Anne B. Lichtenwalner - UMaine Aquatic Animal Health Lab • Kathy M. Castro - University of Rhode Island • Mark D. Fast - Stony Brook University • Roxanna M. Smolowitz - Roger Williams University

  3. Lobster Health Coalition

  4. Data Collection • Summer 2009: • Target: 1500 lobsters from Ventless Trap surveys in 3 general NE regions • Sites: • Maine • Rhode Island/Massachusetts • Long Island Sound (LIS) • Numbers: • Maine: 378 • RI/MASS: 400 • LIS: 154 (VTS animals returned to stock)

  5. Processing Samples

  6. Data Processing • Logged in: • General observations • Size • Vigor/mortality • Visible shell lesions

  7. Data Processing • Hemolymph • Direct observation for parasites • Culture • Pelleted hemocytes for cDNA

  8. Sample Repository • Archival information: • Region/GIS/Date of collection • Hemocyte RNA: • High proportion with high sample integrity • Re-precipitating/cleaning • Histology: • Eyestalk, antennal gland, hepatopancreas, heart, stomach, muscle, gill, gonad, nerve, intestine, shell (formalin fixed, some sectioned) • Toxicology • Hepatopancreas (frozen)

  9. Sample Repository • Total= 932 • Maine: 378 • LIS: 154 • RI/MASS: 400 • Histology prepared/read (R. Smolowitz) • Maine: 35 • LIS: 15 • RI/MASS: 42

  10. .

  11. Bacteremia • Gaffkemia: Aerococcus viridans • None detected • Bumper car disease: 1 • Anophryoides haemophilia

  12. Isolates Identified • Photobacterium spp. • P. indicum • P. damsellae ss damsellae • Other • Pasteurella multocida • Actinomyces • Aeromonas • Listonella anguillarum • Vibrio spp. • V. splendidus • V. aestaurianus • V. alginolyticus • V. carcarias • V. fluvialis • V. furnissii • V. metschnikovii • V. proteolyticus • V. vulnificans • V. harveyii

  13. Further Work • Website development • Grants from • Atlantic Lobster Sustainability Foundation • Margaret E. Burnham Charitable Trust • Northeast AgEnhancement • Repository extension • Expand Lobster Health Coalition

  14. Questions welcomed. Thank you.

  15. The Value of “Value-Added” Sustaining our Lobstermen

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