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Micropipettes. Measuring volumes which are what we scientists call “very, very small”. MICROPIPETTE RULES. Don’t set the pipettes past their intended range (high or low), even though you can – set the pipette volume only within the range specified for that micropipette

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micropipettes

Micropipettes

Measuring volumes which are what we scientists call

“very, very small”

slide3

MICROPIPETTE RULES

  • Don’t set the pipettes past their intended range (high or low), even though you can – set the pipette volume only within the range specified for that micropipette
  • P-20 range is 2 – 20 ul
  • P-100 range is 20 – 100 ul
  • P-200 range is 20 – 200 ul
  • P-1000 range is 200 – 1000 ul
slide4

When using a micropipette, first apply a tip.

  • Check to be sure the tip is on securely.
  • Only open the tip box when getting a tip – DON’T LEAVE IT OPEN!
slide5

NEVER hold pipette so that the tip is higher than the handle or liquid leaks into the barrel (BAD!)

  • Always hold pipettes vertically
  • when in use.
slide6

Feel the stops before you begin taking up or dispensing any liquid.

1st stop: Exact volume to which the pipette is set. Press to this stop before immersing pipette tip in the desired solution.

2nd stop: Use when dispensing solution. Pushes an extra volume of air to dislodge any solution adhering to the tip.

slide8

Extract and dispense SLOWLY.

  • When taking up or dispensing
  • liquid, don’t put the tip any deeper
  • into the liquid than necessary.
  • When dispensing, touch the tip
  • to the side or bottom of the tube,
  • or if there is liquid already in the
  • tube, touch the tip to the liquid.
slide9

Mid-range:

2 - 20 µL

10 -100 µL

20 - 200 µL

Range is printed on the button or on the side

Use medium sized yellow tips

slide10

We will be using P-20 and P-200 pipettes

P-20 has a range of 2 - 20 µL

Digits represent

Tens (10’s)

Ones (1’s)

Tenths (1/10’s)

slide11

10 -100 µL

This pipette is set to its maximum:

100 µL

Digits represent:

100’s

10’s

1’s

slide12

High Range: 200-1000 µL

Use large blue tips

Digits from top to bottom represent:

1000’s

100’s

10’s

The 1’s are indicated by the tiny white tick marks in the dial with the 10’s. Marks are in increments of 2 µL

This pipette is set to 1000 µL