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40 µm

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40 µm

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  1. A B C * * * * * * Supplementary Figure:Double fluorescence staining for neurofilament (RT97, blue) and 3 (red). It can be seen that the 3 ring is around a subpopulation of RT97 positive large neurons. The field was chosen because it had many large neurons; two neurofilament poor (presumably C-fibre neurons) are indicated with asterisks. Primary antibodies as in Methods, secondary antibodies are: for RT97: horse anti-mouse AMCA (1:200), and for 3: donkey anti-rabbit Alexa 594 (1:400). 40 µm

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