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Hardy-Weinberg Lab. Taste Testing. PTC. Phenylthiocarbamide Compound contains sulfur Harmless chemical Found in Brassica family of plants Cabbages, brussel sprouts, kale Gives a slight bitterness. Tasting PTC. Dominant: Tasters Find low concentrations of PTC extremely bitter

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Hardy weinberg lab

Hardy-Weinberg Lab

Taste Testing


PTC

  • Phenylthiocarbamide

    • Compound contains sulfur

    • Harmless chemical

  • Found in Brassica family of plants

    • Cabbages, brussel sprouts, kale

    • Gives a slight bitterness


Tasting ptc
Tasting PTC

  • Dominant: Tasters

    • Find low concentrations of PTC extremely bitter

    • Homozygous tasters seem to have a very low tolerance

      • Find it more bitter than heterozygous tasters

      • Oddity: you can only taste it if it is dissolved in your own saliva

  • Recessive: Non-tasters

    • Report little or no taste


How does this reflect on role of alleles
How does this reflect on role of alleles?

  • Alleles are different versions of same gene.

  • Gene encodes a protein receptor for PTC

    • Found on chromosome 7

  • Tasters have a gene that encode

    • Proline, Alanine and Valine at positions 49, 262 and 296

  • Non-tasters have a gene

    • A, V and I at these three positions


Sodium benzoate
Sodium benzoate

  • Poly-unsaturated fat

  • Used in food to inhibit microbial growth

  • Works best in an acidic environment


Relation to a person s fave foods

PTC bitter/Sodium benzoate salty

Tend to like sauerkraut, buttermilk, turnips and spinach more than average

PTC bitter/Sodium benzoate bitter

Tend to dislike sauerkraut, buttermilk, turnips, spinach less than average

Relation to a person’s fave foods


Most common taste groups
Most common taste groups

  • Ranked in order of most common combos

    PTCSodium Benzoate

  • 1. Bitter salty

  • 2. Bitter sweet

  • 3. Bitter Bitter

  • 4. Tasteless Salty

  • 5. Any other combinations


Lab methods
Lab Methods

  • Part I: PTC Taste/NonTaste

  • Step 1: Place control paper on tongue/chew a little

    • Negative control: you shouldn’t taste anything

    • Something to compare the other papers to…

  • Step 2: Place PTC(blue paper) on tongue

    • Record what you taste

    • If you don’t taste anything, chew it a little and record again

  • Are you a taster or a non-taster?


Lab methods pt 1
Lab Methods…pt 1

  • Step 3: Record your PTC data on the board and collect all class data

  • Step 4: Calculate the allele frequencies and the genotype frequencies of the class


Lab methods pt 2
Lab Methods pt 2

  • thiourea

  • How many of you can taste one but not the other?


Lab methods pt 3
Lab Methods: Pt 3

  • Sodium benzoate


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