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Hardy-Weinberg Lab. Taste Testing. PTC. Phenylthiocarbamide Compound contains sulfur Harmless chemical Found in Brassica family of plants Cabbages, brussel sprouts, kale Gives a slight bitterness. Tasting PTC. Dominant: Tasters Find low concentrations of PTC extremely bitter

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hardy weinberg lab

Hardy-Weinberg Lab

Taste Testing

slide2
PTC
  • Phenylthiocarbamide
    • Compound contains sulfur
    • Harmless chemical
  • Found in Brassica family of plants
    • Cabbages, brussel sprouts, kale
    • Gives a slight bitterness
tasting ptc
Tasting PTC
  • Dominant: Tasters
    • Find low concentrations of PTC extremely bitter
    • Homozygous tasters seem to have a very low tolerance
      • Find it more bitter than heterozygous tasters
      • Oddity: you can only taste it if it is dissolved in your own saliva
  • Recessive: Non-tasters
    • Report little or no taste
how does this reflect on role of alleles
How does this reflect on role of alleles?
  • Alleles are different versions of same gene.
  • Gene encodes a protein receptor for PTC
    • Found on chromosome 7
  • Tasters have a gene that encode
    • Proline, Alanine and Valine at positions 49, 262 and 296
  • Non-tasters have a gene
    • A, V and I at these three positions
sodium benzoate
Sodium benzoate
  • Poly-unsaturated fat
  • Used in food to inhibit microbial growth
  • Works best in an acidic environment
relation to a person s fave foods
PTC bitter/Sodium benzoate salty

Tend to like sauerkraut, buttermilk, turnips and spinach more than average

PTC bitter/Sodium benzoate bitter

Tend to dislike sauerkraut, buttermilk, turnips, spinach less than average

Relation to a person’s fave foods
most common taste groups
Most common taste groups
  • Ranked in order of most common combos

PTCSodium Benzoate

  • 1. Bitter salty
  • 2. Bitter sweet
  • 3. Bitter Bitter
  • 4. Tasteless Salty
  • 5. Any other combinations
lab methods
Lab Methods
  • Part I: PTC Taste/NonTaste
  • Step 1: Place control paper on tongue/chew a little
    • Negative control: you shouldn’t taste anything
    • Something to compare the other papers to…
  • Step 2: Place PTC(blue paper) on tongue
    • Record what you taste
    • If you don’t taste anything, chew it a little and record again
  • Are you a taster or a non-taster?
lab methods pt 1
Lab Methods…pt 1
  • Step 3: Record your PTC data on the board and collect all class data
  • Step 4: Calculate the allele frequencies and the genotype frequencies of the class
lab methods pt 2
Lab Methods pt 2
  • thiourea
  • How many of you can taste one but not the other?
lab methods pt 3
Lab Methods: Pt 3
  • Sodium benzoate
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