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Exp. ,,6,, Enzymatic digestion of fat by pancreatic lipase

Introduction: Fats (lipid) : consist of a wide group of compounds that are generally soluble in organic solvents and largely insoluble in water. Chemically , fats are generally triesters of glycerol and fatty acids. Exp. ,,6,, Enzymatic digestion of fat by pancreatic lipase.

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Exp. ,,6,, Enzymatic digestion of fat by pancreatic lipase

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  1. Introduction: Fats (lipid) : consist of a wide group of compounds that are generally soluble in organic solvents and largely insoluble in water. Chemically, fats are generally triesters of glycerol and fatty acids Exp. ,,6,,Enzymatic digestion of fat by pancreatic lipase

  2. A triglyceride consists of a glycerol and three fatty acids. It is the major type of lipid used for energy storage... • Fatty Acids:The lipid building blocks.

  3. Lipases: are enzymes which hydrolyse the triglycerides, releasing fatty acids. • +3H₂O lipase glycerol + f.a

  4. Triglycerides structure:

  5. The hydrolysis process can give all three fatty acids or it can yield one or two F.AS with diglycerides and monoglycerides... • FA1 FA2 FA3 • FA2 Lipase + Lipase FA FA1 • FA3 FA2

  6. Materials: • Fresh whole milk • Litmus solution ( or litmus paper) as an indicator. • Pancreatin solution (boiled, unboiled). • 1% Calcium hydroxide solution.

  7. Procedure: • 4ml milk half of A+ • + 10 drops 1ml boiled Litmus + 3 drops pancreatin so Ca(OH) 2 + 1ml unboiled in water bath (45⁰c) for4-6 hours.

  8. Results: • Accumulation of free fatty acids will reduce the PH. • Pink colour with indicator • Odour • Precipitate.

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