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RESPIRATION LAB

RESPIRATION LAB. What is the Effect of Body Mass on the Rate of Oxygen Consumption. Instructor Terry Wiseth. Click to go to Next Slide.

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RESPIRATION LAB

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  1. RESPIRATION LAB What is the Effect of Body Mass on the Rate of Oxygen Consumption Instructor Terry Wiseth Click to go to Next Slide

  2. Most cells consume oxygen and produce carbon dioxide. The higher the rate of metabolism, the more oxygen used by the cells, and by the animal as a whole. One way to determine the basal metabolic rate (BMR), therefore, is to measure the amount of oxygen consumed by the animal. In general, larger animals consume more oxygen than smaller animals. Rubner's Surface Rule maintains that the metabolic rate is affected by the ratio of surface area to volume. According to this theory, cells in smaller animals maintain a higher metabolic rate to compensate for the greater amount of heat lost from their bodies. Click on the blackboard here to zoom in on a larger blackboard Click to go to Next Slide

  3. However, the same relationship between size and metabolic rate has been found in cold-blooded animals. These animals have body temperatures similar to the environment and do not compensate for heat loss. Clearly, other factors must be at work here. While you may predict that a larger animal will consume more oxygen than a smaller animal, the situation may be more complex if you compare the BMR (Basic Metabolic Rate) per gram of two animals. In this lab, the BMR’s of white rats of varying weights are determined by measuring their rates of oxygen consumption. Click to go to Next Slide

  4. This data is then used to calculate oxygen consumption per gram of body weight, and the results are graphed to allow comparison of oxygen/minute vs. oxygen/hour/gram.Using this information you should be able to draw conclusions about the effect of body mass on the metabolic rate. In this simulation the rate of oxygen consumption is measured from laboratory rats. You will determine the basal metabolic rate for each rat and calculate the rate of oxygen consumed by a gram of tissue. In this way the effect of body mass on oxygen consumption and on oxygen consumption per gram is determined. Click to go to Next Slide

  5. In the traditional wet lab a weighed rat is placed in a wire cage which is placed in a chamber containing soda lime. The chamber is sealed using a cork and soap bubbles are placed in the end of the calibrated tube. In this system the rat consumes oxygen and the soda lime absorbs exhaled carbon dioxide. Thus, as oxygen is used, the volume of air in the chamber decreases, and the bubbles move down the tube. The locations of the bubbles in the tube is measured every 15 minutes and the rate of oxygen consumption is calculated. Click to go to Next Slide

  6. This lab will require that you collect data using provided data sheets and analyze the data by producing bar graphs. (Use the links below to print the tables and graphs) 1) Four Table 1 Data Sheets (1 for each Rat) 2) Table 2 Data Sheet 3) Bar Graph 1 4) Bar Graph 2 5) Questions O2 Consumption 1-3 Click Here to View Instructions on how to construct the graphs Click to go to Next Slide If you are done with this lab click here

  7. When you are finished with this lab, be sure that you have included in your lab report the following: (Use the Links below to print the tables and graphs) 1) Four Table 1 Data Sheets (1 for each Rat) 2) Table 2 Data Sheet 3) Bar Graph 1 4) Bar Graph 2 5) Answers to questions 1-3 Click Here to View Instructions on how to construct the graphs Click to Start the Experiment If you are done with this lab click here

  8. :00 There are four rats in the rack. Select one of the rats by clicking on it. The rat will be weighed on the scale in grams. Record the weight of the rat in Data Table 1. You will be using all four of the rats in your experiment. If you have performed the experiment with all of the rats click here

  9. Mabel

  10. :00 There are four rats in the rack. Select one of the rats by clicking on it. The rat will be weighed on the scale in grams. Record the weight of the rat in Data Table 1 You have chosen Mabel 32.42 g Click to go to Next Slide

  11. :00 After you have recorded the weight, and the name of the subject, click on Mabel to insert her into the wire cage inside the metabolic chamber. 32.42 g Click to go to Next Slide

  12. 10 1 2 3 4 5 6 7 8 9 :00 Locate the beaker containing the bubble solution and pipette. Click on the blue pipette to inject the bubbles into the end of the tube leading from the respiratory chamber. 32.42 g Click to go to Next Slide

  13. 10 1 2 3 4 5 6 7 8 9 :00 Measure the location of the extreme right side of the bubble in the tube. Record the value in Data Table 1at TIME 0:00. (The value recorded should be 10) 32.42 g Click to go to Next Slide

  14. 10 1 2 3 4 5 6 7 8 9 Click the Start Timer button for the timer to initiate the experiment. You will need to record where the edge of the bubble is at every 1 minute interval for 6 minutes. Record these values in Data Table 1 6:00 5:00 :30 1:00 1:30 2:00 3:30 3:00 4:00 4:30 5:30 2:30 :00 Start Timer The Timer has startedWait for each 1 minute reading on the clock above to record your data 32.42 g Stop the Timer and return to select a different Rat Click to go to Next Slide

  15. Josie

  16. :00 There are four rats in the rack. Select one of the rats by clicking on it. The rat will be weighed on the scale in grams. Record the weight of the rat in Data Table 1 You have chosen Josie 12.0 g Click to go to Next Slide

  17. :00 After you have recorded the weight, and the name of the subject, click on Josie to insert her into the wire cage inside the metabolic chamber. 12.0 g Click to go to Next Slide

  18. 10 1 2 3 4 5 6 7 8 9 :00 Locate the beaker containing the bubble solution and pipette. Click on the blue pipette to inject the bubbles into the end of the tube leading from the respiratory chamber. 12.0 g Click to go to Next Slide

  19. 10 1 2 3 4 5 6 7 8 9 :00 Measure the location of the extreme right side of the bubble in the tube. Record the value in Data Table 1at TIME 0:00. (The value recorded should be 10) 12.0 g Click to go to Next Slide

  20. 10 1 2 3 4 5 6 7 8 9 Click the Start Timer button for the timer to initiate the experiment. You will need to record where the edge of the bubble is at every 1 minute interval for 6 minutes. Record these values in Data Table 1 6:00 5:00 :30 1:00 1:30 2:00 3:30 3:00 4:00 4:30 5:30 2:30 :00 Start Timer The Timer has startedWait for each 1 minute reading on the clock above to record your data 12.0 g Stop the Timer and return to select a different Rat Click to go to Next Slide

  21. Tex

  22. :00 There are four rats in the rack. Select one of the rats by clicking on it. The rat will be weighed on the scale in grams. Record the weight of the rat in Data Table 1 You have chosen Tex 42.89 g Click to go to Next Slide

  23. :00 After you have recorded the weight, and the name of the subject, click on Tex to insert her into the wire cage inside the metabolic chamber. 42.89 g Click to go to Next Slide

  24. 10 1 2 3 4 5 6 7 8 9 :00 Locate the beaker containing the bubble solution and pipette. Click on the blue pipette to inject the bubbles into the end of the tube leading from the respiratory chamber. 42.89 g Click to go to Next Slide

  25. 10 1 2 3 4 5 6 7 8 9 :00 Measure the location of the extreme right side of the bubble in the tube. Record the value in Data Table 1at TIME 0:00. (The value recorded should be 10) 42.89 g Click to go to Next Slide

  26. 10 1 2 3 4 5 6 7 8 9 Click the Start Timer button for the timer to initiate the experiment. You will need to record where the edge of the bubble is at every 1 minute interval for 6 minutes. Record these values in Data Table 1 6:00 5:00 :30 1:00 1:30 2:00 3:30 3:00 4:00 4:30 5:30 2:30 :00 Start Timer The Timer has startedWait for each 1 minute reading on the clock above to record your data 42.89 g Stop the Timer and return to select a different Rat Click to go to Next Slide

  27. Gertie

  28. :00 There are four rats in the rack. Select one of the rats by clicking on it. The rat will be weighed on the scale in grams. Record the weight of the rat in Data Table 1 You have chosen Gertie 22.92 g Click to go to Next Slide

  29. :00 After you have recorded the weight, and the name of the subject, click on Gertie to insert her into the wire cage inside the metabolic chamber. 22.92 g Click to go to Next Slide

  30. 10 1 2 3 4 5 6 7 8 9 :00 Locate the beaker containing the bubble solution and pipette. Click on the blue pipette to inject the bubbles into the end of the tube leading from the respiratory chamber. 22.92 g Click to go to Next Slide

  31. 10 1 2 3 4 5 6 7 8 9 :00 Measure the location of the extreme right side of the bubble in the tube. Record the value in Data Table 1at TIME 0:00. (The value recorded should be 10) 22.92 g Click to go to Next Slide

  32. 10 1 2 3 4 5 6 7 8 9 Click the Start Timer button for the timer to initiate the experiment. You will need to record where the edge of the bubble is at every 1 minute interval for 6 minutes. Record these values in Data Table 1 6:00 5:00 :30 1:00 1:30 2:00 3:30 3:00 4:00 4:30 5:30 2:30 :00 Start Timer The Timer has startedWait for each 1 minute reading on the clock above to record your data 22.92 g Click to go to Next Slide Stop the Timer and return to select a different Rat

  33. Questions

  34. Construct Bar Graphs From the data you have collected and calculated, construct bar graphs which compare the weights of different sized rats and their oxygen consumption. Use Bar Graph 1to compare Average Milliliters of Oxygen consumed per Minute vs the Weight of the individual animals. Use Bar Graph 2to compare the Average Milliliters of Oxygen consumed per Hour per Gram vs the Weight of the individual animals. Formulas for manipulating the data are given in Table 2. :00 Click to go to Next Slide

  35. Answer the Following Questions • Is there any relationship between the values for oxygen consumed / minute for each animal and the weight? • 2) Is there any relationship between the values for oxygen consumed / hour / gram for each animal and the weight? • 3) Why would a small rat have a different rate of oxygen consumption than a larger animal? :00 Click Here to View a Printable Page of the Questions Click to go to Next Slide

  36. Data Table and Graphs

  37. Click to View A Printable Page DATA TABLE 1 10 Click to go back to last slide viewed

  38. Click to View A Printable Page DATA TABLE 2 How to calculate Avg O2 / minute Total difference divided by 6 How to calculate Avg O2 / hour / g Avg O2 / minute times 60 divided by weight of rat Click to go back to last slide viewed

  39. Click to View A Printable Page BAR GRAPH 1 2 - 1.5 - 1 - 0.5 - 0 - 12.0 22.92 32.42 42.89 Click to go back to last slide viewed

  40. Click to View A Printable Page BAR GRAPH 2 4 - 3 - 2 - 1 - 0 - 12.0 22.92 32.42 42.89 Click to go back to last slide viewed

  41. 10 1 2 3 4 5 6 7 8 9 :00 42.89 g

  42. 10 1 2 3 4 5 6 7 8 9 :00 42.89 g

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