Pakistan barcode project progress and applications
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Pakistan barcode project: progress and applications. Muhammad Ashfaq. 2010: Barcoding economically important insect species from Pakistan. Biodiversity Institute of Ontatio. National Institute for Biotechnology and Genetic Engineering (NIBGE). Funded by: HEC. Project execution.

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Pakistan barcode project: progress and applications

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Pakistan barcode project:progress and applications

Muhammad Ashfaq


2010: Barcoding economically important insect species from Pakistan

Biodiversity Institute of Ontatio

National Institute for Biotechnology and Genetic Engineering (NIBGE)

Funded by: HEC


Project execution


Activities and Progress

Shaking onto sheets

Light traps

Arial nets

Manual catching

Pipettes and sieves

Collecting animal parasites


Specimen preparation and databasing


Specimens

Animals: 34103

Plants: 1289

Barcodes

Animals:28605

Plants: 988

18 months

IDRC (62%)


Beneficials

Pests


II.Plants: 363

Singletons: 1530

Number of BINs/ species

Pakistan-only BINs/ species


II.Genera: 698

Unidentified: 22308

III.Species: 764

Taxonomic identifications


Barcode Applications


Reference libraries


Helicoverpa armigera

98

33

Helicoverpa zea (GU087831)

Heliothinae

Heliothis virescens (GU336572)

73

Masalia sp.

Heliothis sp.

21

24

Heliocheilus confertissima

21

Spodoptera littoralis

90

Spodoptera litura

Amphipyrinae

23

Spodoptera cilium

90

Spodoptera exigua

53

Mamestra sp.

Mythimna sp.

Hadeninae

19

5

Leucania venalba

18

Mythimna convecta

41

Agrotis segetum

92

Noctuinae

Agrotis ipsilon

Chrysodeixis acuta

2

49

Thysanoplusia orichalcea

Plusiinae

91

Chrysodeixis eriosoma

41

Ctenoplusia albostriata

30

Trichoplusia ni

29

Hypocala deflorata

Catocalinae

Pandesma robusta

86

Dysgonia torrida

72

Achaea janata

89

Earias insulana

Chloephorinae

Earias vittella

99

Gelechiidae

Pectinophora gossypiella

Noctuid species in/around cotton fields of Punjab

Previously only S. litura was reported from cotton in Punjab


Genetic diversity: mango mealybug, Drosichamangiferae


91

Bt

-

1 (36) [AAM1247]

Asia II 1

99

40

Bt

-

2 (1) [ AAM1246]

84

Bt

-

3 (2) [ACB7718]

29

99

Bt

-

4 (5) [

AAM1248]

Asia II 7

63

99

Bt

-

5 (2)

98

58

Bt

-

6 (8) [AAM1243]

99

52

Bt

-

7 (11) [AAM1245]

Asia II 5

99

Bt

-

8 (15) [AAM1244]

Asia 1

99

99

Bt

-

9 (2)

59

Bt

-

10 (2) [AAA4495]

99

99

Bt

-

11 (13) [AAT8875]

MEAM1

97

Bt

-

12 (19)

99

0.02

Bemisia tabaci, a complex of cryptic species


Bemisia tabaci, distribution analysis

(A) Asia II 1

(B) MEAM 1

(C) Asia 1


Dengue epidemic in Punjab


Mosquito species in Punjab


Genus-wise distribution of mosquitoes in Punjab

Genus: Aedes

Aedes aegypti

Aedes albopictus

Genus: Anopheles

Genus: Culex


Outreach


Barcoding project at NIBGE


Barcoding team members


Thank you for your attention


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