Supplemental Figure 3
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Supplemental Figure 3. Ngn3. HES1. Hexokinase 2. 4. 2.5. 8. 2. 3. 6. 1.5. 4. 2. 1. 2. 1. 0.5. 0. 0. 0. Ngn3-EGFP +. Ngn3-EGFP -. Ngn3-EGFP +. Ngn3-EGFP -. Ngn3-EGFP +. Ngn3-EGFP -. Insulin I. Pdx-1. MafA. 15. 2. 3. 1.5. 10. 2. 1. 5. 1. 0.5. 0. 0. 0.

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Supplemental Figure 3

Ngn3

HES1

Hexokinase 2

4

2.5

8

2

3

6

1.5

4

2

1

2

1

0.5

0

0

0

Ngn3-EGFP+

Ngn3-EGFP-

Ngn3-EGFP+

Ngn3-EGFP-

Ngn3-EGFP+

Ngn3-EGFP-

Insulin I

Pdx-1

MafA

15

2

3

1.5

10

2

1

5

1

0.5

0

0

0

Ngn3-EGFP+

Ngn3-EGFP-

Ngn3-EGFP+

Ngn3-EGFP-

Ngn3-EGFP+

Ngn3-EGFP-

Glucokinase

Glut2

Pax4

Relative Expression

2.5

2

1.5

2

1.5

1.5

1

1

1

0.5

0.5

0.5

0

0

0

Ngn3-EGFP+

Ngn3-EGFP-

Ngn3-EGFP+

Ngn3-EGFP-

Ngn3-EGFP+

Ngn3-EGFP-

Pax6

Supplemental Figure 3. Expression of genes in sorted Ngn3-EGFP+ or Ngn3-EGFP- populations isolated from day 10 of tertiary culture. Amplified cDNA samples used for PancChip analysis were analyzed by real time quantitative PCR.

2.5

2

1.5

1

0.5

0

Ngn3-EGFP+

Ngn3-EGFP-


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