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Principles of genetic engineering. L Mathias. What is genetic engineering. Genetic engineering, also known as recombinant DNA technology , means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype.

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Principles of genetic engineering

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Principles of genetic engineering l.jpg

Principles of genetic engineering

L Mathias


What is genetic engineering l.jpg

What is genetic engineering

  • Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype.

  • Various kinds of genetic modification are possible: inserting a foreign gene from one species into another, forming a transgenic organism; altering an existing gene so that its product is changed; or changing gene expression so that it is translated more often or not at all.


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Basic steps in genetic engineering

  • Isolate the gene

  • Insert it in a host using a vector

  • Produce as many copies of the host as possible

  • Separate and purify the product of the gene


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Step 1: Isolating the gene


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Step 1: Alternative method (using reverse transcriptase)

  • Reverse transcriptase

  • mRNA converted into cDNA

  • Complementary strand produced using DNA polymerase

  • Advantage – more mRNA in cell than DNA


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Step 2: Inserting gene into vector

  • Vector – molecule of DNA which is used to carry a foreign gene into a host cell


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Step 3: inserting vector into host

  • See worksheet


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Replica plating


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Step 4: Multiplication of the host cells by cloning

  • Large scale fermenters by cloning

  • All genetically identical because of asexual reproduction


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Step 5: Extraction of desired gene product


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