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Spinal Cord Injury. Topics in Bioengineering. http://neuroscience.uth.tmc.edu/s2/chapter03.html. Characterization of the cell populations in vitro. bFGF. BMP-4. CNTF. Nuclei GFAP (differentiation) Olig2 (injury). Markers of astrocyte differentiation/ astroglial lineage.

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Spinal cord injury

Spinal Cord Injury

Topics in Bioengineering



Characterization of the cell populations in vitro
Characterization of the cell populations in vitro

bFGF

BMP-4

CNTF

Nuclei

GFAP (differentiation)

Olig2 (injury)

Markers of astrocyte differentiation/ astroglial lineage

Davies et al (2011) PLoS One. Figure 1


Characterization of the cell populations in vitro1
Characterization of the cell populations in vitro

9W-1

9W-2

Glial scar formation/ injury response

Davies et al (2011) PLoS One. Figure 1


Transplant morphology human gda survival and support of axon growth within dlf injuries
Transplant morphology: Human GDA survival and support of axon growth within DLF injuries

NF+ density:

BMP: 14.79 ± 3.05

CNTF: 7.75 ± 2.28

Davies et al (2011) PLoS One. Figure 2 and Suppl Figure 1; http://vanat.cvm.umn.edu/neurLab2/SpCdWhite.html


Cell migration and gfap staining within injuries
Cell migration and GFAP staining within injuries axon growth within DLF injuries

Arrowheads indicate cell bodies

Max rostral/caudal migration:

CNTF: 3.24/ 3.96 mm

BMP: 2.52/ 2.16 mm

Davies et al (2011) PLoS One. Figure 3


Functional assessment after implantation of hgdas bmp hgdas cntf or hgcps
Functional assessment after implantation of axon growth within DLF injurieshGDAsBMPhGDAsCNTF or hGCPs

Grid walk test: http://www.pnas.org/content/suppl/2007/02/19/0611723104.DC1/11723Movie1.mov

Davies et al (2011) PLoS One. Figure 5


Neuroprotection and survival
Neuroprotection axon growth within DLF injuries and survival

White = NeuN (neuron survival marker)

Davies et al (2011) PLoS One. Figures 6 and 7


The injury model
The Injury Model axon growth within DLF injuries

Van den Brand et al (2012) Science. Suppl Figure 1


The robotic postural interface
The Robotic Postural Interface axon growth within DLF injuries

Van den Brand et al (2012) Science. Suppl Figure 2


Multi system neuroprosthetic training system
Multi-System Neuroprosthetic Training System axon growth within DLF injuries

Van den Brand et al (2012) Science. Suppl Figure 3


Locomotion after training
Locomotion after training axon growth within DLF injuries

Van den Brand et al (2012) Science. Figure 1


Locomotion after training1
Locomotion after training axon growth within DLF injuries

http://www.sciencemag.org/content/suppl/2012/05/30/336.6085.1182.DC1/1217416s1.mov

Van den Brand et al (2012) Science. Supplementary Movie 1


Neural circuit remodeling
Neural circuit remodeling axon growth within DLF injuries

Van den Brand et al (2012) Science. Figure 2


Neural circuit remodeling1
Neural circuit remodeling axon growth within DLF injuries

Van den Brand et al (2012) Science. Figure 3


Neural circuit remodeling2
Neural circuit remodeling axon growth within DLF injuries

Van den Brand et al (2012) Science. Figure 4


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