This lab was originally designed by students at mit
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This lab was originally designed by students at MIT. Bioengineering: Banana smell generating device. E. coli with foul smell (HB101). Delete the tnaA5 gene that produces indole (odor) . E. coli with less odor (YYC912). Add the ATF1 gene. E. coli with ATF1.

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Bioengineering: Banana smell generating device

E. coli with foul smell (HB101)

Delete the tnaA5 gene that produces indole (odor)

E. coli with less odor (YYC912)

Add the ATF1 gene

E. coli with ATF1

Add the precursor isoamyl alcohol

E. coli that smell like bananas


Banana smell generating device

ATF1

Isoamyl

alcohol

Isoamyl Acetate

(banana smell)

  • ATF1: encodes Alcohol acetyltransferasethat converts isoamyl alcohol to isoamyl acetate. Derived from yeast.

  • Isoamyl alcohol: a precursor for the enzyme ATF1. Nearly odorless. Needs to be added to the culture to obtain banana smell.

  • Isoamyl acetate: the product of the reaction. This is the chemical that is naturally produces by bananas to produce their smell.


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