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Holliday model

Holliday model. Strand exchange and Heteroduplex DNA formation. HJ resolution. Mismatch repair. Meselson-Radding 1975. Szostak Orr-Weaver Rothstein Stahl (1983) model. Synthesis Dependent Strand Annealing. Rad51-dependent ectopic (interchromosomal) gene conversion in haploid cells.

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Holliday model

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  1. Holliday model Strand exchange and Heteroduplex DNA formation HJ resolution Mismatch repair

  2. Meselson-Radding 1975

  3. Szostak Orr-Weaver Rothstein Stahl (1983) model

  4. Synthesis Dependent Strand Annealing

  5. Rad51-dependent ectopic (interchromosomal) gene conversion in haploid cells HOendonuclease-induced DSB Non-crossover Crossover

  6. wild type noncrossover crossover crossover noncrossover Crossovers and Noncrossovers occur with different kinetics Analogous results in yeast meiosis (Allers and Lichten 2001)

  7. Modified SDSA SzOrRoST (1983)

  8. Spo11 Mre11 Rad50 etc Meiotic recombination Axial element DSB Synaptonemal complex Timing of meiotic DSBs: 2 hr after replication. Suggests some structures are assembled during replication that are needed to create DSB

  9. Kinetics of meiotic recombination initiated by a DSB Double HJ 2D gels 2D gels, as used to examine replication

  10. Analysis of Joint Molecule intermediates by denaturing gel electrophoresis Is it this ? Or this? This

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