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1077102

0301


Background

BACKGROUND

  • 1985WHO


Background1

BACKGROUND

  • NO


Background2

BACKGROUND

  • organ bath


Objective

OBJECTIVE

  • or

  • Ryanodine IP3


Methods

METHODS

SD

organ bath


Methods1

METHODS

PEKCl

  • K-H 34 mm8 K-H 3, 1, 1.5, 2, 2.5, 3, 3.5, 4 g 60 min


Methods2

METHODS

  • KCl 0.06mol/L KCl 3

    10-6 mol/LPE 10-5 mol/LACh 50%50%


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PE

10-6 mol/L

5 min

0.1 3

5 min

0.1 3

KCl

610-2 mol/L

METHODS

  • PEKCl

  • PEKCl


Methods3

METHODS

CaCl2

  • 3 g

  • K-H 3 30 min K-H 0.06 mol/L KCl10-6 mol/LPE 5 minCaCl20.255 mmol/L


Methods4

METHODS

KCl

KCl+

PE

PE+

K-H

K-H

K-H

K-H

3

3

30 min

30 min

30 min

30 min

K-H

K-H

K-H

K-H

KCl

0.06 mol/L

KCl

0.06 mol/L

PE

10-6 mol/L

PE

10-6 mol/L

5 min

CaCl20.25-5 mmol/L

5 min

CaCl20.25-5 mmol/L

5 min

CaCl20.25-5 mmol/L

5 min

CaCl20.25-5 mmol/L


Methods5

METHODS

verapamil, Ver

  • 3 g


Methods6

METHODS

  • 1Ver10-6 mol/LLVer30 minPE10-6 mol/L20 min

    2Ver+10-6 mol/LVer30 minPE10-6 mol/L3 20 min

    3Ver+RR+10-6 mol/LVer5 min10-5 mol/Lryanodineruthenium red, RR25 minPE10-6 mol/L320 min

    4Ver+HP+10-6 mol/LVer5 min50 mg/LIP3heparin, HP25minPE10-6 mol/L3 20 min


Methods7

10 min

METHODS

Ver (10-6 mol/L) 30 min

PE (10-6 mol/L) 30 min

Ver

320 min

Ver+

320 min

RR 10-5mol/L

Ver+RR+

5 min

10 min

320 min

HP 50mg/L

Ver+HP+

5 min

10 min


Results

RESULTS

KCl

Fig. 1A. Concentration-dependent effect of ethanol (0.1-3) in 6102 M KCl precontracted rat aortic rings with endothelium at different resting tension (1 g-4 g). Data are expressed as Mean SD (n=3-5). * P<0.05, vs. Control-3 g.


Results1

RESULTS

Fig. 1B. Concentration-dependent effect of ethanol (0.1-3) in 6102 M KCl precontracted rat aortic rings without endothelium at different resting tension (1 g-4 g). Data are expressed as Mean SD (n=3-5). * P<0.05, vs. Control-3 g.


Results2

RESULTS

PE

Fig. 2A. Concentration-dependent effect of ethanol (0.1-3) in 10-6 M PE precontracted rat aortic rings with endothelium at different resting tension (1 g-4 g).Data are expressed as Mean SD (n=3-6).


Results3

RESULTS

Fig. 2B. Concentration-dependent effect of ethanol (0.1-3) in 10-6 M PE precontracted rat aortic rings without endothelium at different resting tension (1 g-4 g).Data are expressed as Mean SD (n=3-6). * P<0.05, ** P<0.01, vs. Control-3 g.


Results4

RESULTS

CaCl2

Fig. 3A. Effect of ethanol at 3 on the Ca2+ (2.510-4-510-3 M)-induced contraction of rat aortic rings (the rest tension was 3 g) without endothelium pretreated with 6102 M KCl. Data are expressed as Mean SD (n=5-6). * P <0.05, ** P <0.01, vs. Control.


Results5

RESULTS

Fig. 3B. Effect of ethanol at 3 on the Ca2+ (2.510-4-510-3 M)-induced contraction of rat aortic rings (the rest tension was 3 g) without endothelium pretreated with 10-6 M PE. Data are expressed as Mean SD (n=5-6). ** P <0.01, vs. Control.


Results6

RESULTS

verapamil, Ver

Ver: only pretreated with verapamil;

Ver+Ethanol: ethanol treatment after

verapamil incubation;

Ver+RR+Ethanol: ethanol treatment

after verapamil and ruthenium red

(RR, 105 M) incubation;

Ver+HP+Ethanol: ethanol treatment

after verapamil and heparin

(HP, 50 mg/L) incubation

Fig. 4. Effect of ethanol at 3 in rat aortic rings (the rest tension was 3 g) without endothelium precontracted with PE (10-6 M) after verapamil (Ver, 10-6 M) pretreatment. Data are expressed as Mean SD (n=5-6).* P<0.05, ** P<0.01, vs. Ver; # P<0.05, vs. Ver+Ethanol.


Conclusion

CONCLUSION

  • 3g

  • IP3Ryanodine


Perspective

PERSPECTIVE

  • NO


Acknowledgement

ACKNOWLEDGEMENT

Acknowledgement Acknowledgement Acknowledgement

Acknowledgement Acknowledgement

Acknowledgement Acknowledgement Acknowledgement

Acknowledgement Acknowledgement

Acknowledgement Acknowledgement

Acknowledgement Acknowledgement

Acknowledgement Acknowledgement Acknowledgement

Acknowledgement Acknowledgement


References

REFERENCES

  • ,,,,.., 2003, 24(24):33212234

  • ,,,,.., 2005, 40(6):427430

  • .., 2003, 28( 11) : 10601062

  • .., 2005, 30(16) :12831286


References1

REFERENCES

  • ..20063512933

  • ..200635(2)178181

  • .().:, 2004:270271

  • ,..:, 2004:285288


References2

REFERENCES

  • CHEN Jing, GU Yong, LIN Fan, YANG Haichun, ZHU Weiyu, MA Ji, LIN Shanyan.Endothelin receptor antagonist com bined with a calcium channel blocker attenuates renal injury in spontaneous hypertensive rats with diabetes.Chinese Medical Journal, 2002, 115(7)972978

  • Mohanty M J, Li X L.Stretch-induced Ca2+ release via an IP3-insensitive Ca2+ channel. [J] Am J Physiol Cell Physiol, 2002, 283: 456462.

  • McCarron J G, Bradley K N, MacMillan D, et al.Sarcolemma agonist-induced interactions between InsP3 and ryanodine receptors in Ca2+ oscillations and waves in smooth muscle.[J] Biochem Soc Tran, 2003, 31 (Pt 5): 920924.

  • Bolton T B, Gordienko D V, Pucovsky V, et al.Calcium release events in excitation-contraction coupling in smooth muscle. [J] Novartis Found Symp, 2002, 246: 154168.


References3

REFERENCES

  • Rembold C M.Regulation of contraction and relaxation in arterial smooth muscle.[J] Hypertension, 1992, 20(2): 129137.

  • LI PL, LEE HC, NELSON MT, et al.Novel Ca2+ signalling mechanisms in vascular myocytes: symposium overview.[J] Acta Physiol Scand, 2003, 179(4): 339352.

  • Jaggar JH, Wellman GC, Heppner TJ, Porter VA, Perez GJ, Gollasch M, Kleppisch T,Rubart M,Stevenson AS, Lederer WJ, Knot HJ, Bonev AD, Nelson MT.Ca2+ channels,ryanodine receptors and Ca(2+)-activated K+ channels: a functional unit for regulating arterial tone.Acta Physiol Scand, 1998;164:577587.


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you


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