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G.M. Cook, J. P. Rothenberger, M. Sikaroodi, E. Peters, P.M. Gillevet, R. B. Jonas - PowerPoint PPT Presentation


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A Comparison of Bacterial Communities Between Geographically Separated Corals Infected with White Plague Type II. G.M. Cook, J. P. Rothenberger, M. Sikaroodi, E. Peters, P.M. Gillevet, R. B. Jonas Department of Environmental Science and Policy, George Mason University Tetra Tech, Inc.

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slide1

A Comparison of Bacterial Communities Between Geographically Separated Corals Infected with White Plague Type II

G.M. Cook, J. P. Rothenberger, M. Sikaroodi,

E. Peters, P.M. Gillevet, R. B. Jonas

Department of Environmental Science and Policy, George Mason University

Tetra Tech, Inc.

introduction
Introduction
  • Acquired samples of apparently healthy and diseased corals (Montastraea annularis) from the U.S. Virgin Islands and the Bahamas
  • Compared the microbial community composition of healthy and infected coral pairs
  • Microbial culturing, LH-PCR, molecular fingerprinting, and 16S rRNA sequencing
slide3

M. faveolata, Andros Island, Bahamas.

July 29, 2002

Photo by: Dr. Robert Jonas

slide4

13.5 cm

M. faveolata, Andros Island, Bahamas.

August 18, 2002 (20 days after initial observation)

Photo by: Dr. Robert Jonas

slide5

M. faveolata, Andros Island, Bahamas.

August 1, 2003 (one year after initial observation)

Photo by: Dr. Robert Jonas

hypotheses
Hypotheses
  • That the causative agents are opportunistic pathogens normally present in the host or its environs rather than a novel, obligate pathogen;
  • That corals exhibiting WPII disease signs from different geographical regions harbor differing microbial communities in normal tissue and diseased tissue;
  • The WPII disease process is the result of a broad shift in the microbial community (dysbiosis).
methodology
Methodology

Photo by: Dr. Robert Jonas

Divers sampling a colony of M. faveolata, LSI, Exumas, Bahamas

lee stocking island exumas bahamas
Lee Stocking Island, Exumas, Bahamas

ggggg

X

X

X

Photo by: Dr. Robert Jonas

st croix u s virgin islands
St. Croix, U.S. Virgin Islands

X

Photo by: Dr. Robert Jonas

experimental design
Experimental Design
  • Two cores used for histological investigations
  • Three cores used for microbial & molecular investigations

1.6 cm

Photo By: Dr. Esther Peters

Photo By: Dr. Robert Jonas

slide13

OTU 1 *

OTU 2 *

OTU 3 *

Relative Intensity

Size(bp)

Length Heterogeneity PCR

Fingerprinting

(LH-PCR)

27F

355R

Peak area ~ Abundance

fingerprint histogram1
Fingerprint Histogram

* Inactive WPII

results conclusions
Results/Conclusions
  • The Virgin Island Control/Healthy community is not nearly as diverse as the Bahamas Control/Healthy community.
  • There is a shift from a low complexity (H, HD) to high complexity community (D) in VI samples.
  • There is a shift from a high complexity (H, HD) to low complexity community (D) in the Bahamas samples.
the clustering of diseased tissue d is different from healthy tissue hd and the control tissue h
The clustering of diseased tissue (D) is different from healthy tissue (HD) and the control tissue (H).

The clustering of the healthy and control tissue from the VI (●) is different from the healthy and control tissue from the Bahamas (▲).

Axis 2

results conclusions1
Results/Conclusions
  • WPII was inactive in VI samples.
  • Possibleetiological agent fingerprint, Aurantimonascoralicida (313.1 bp), was found only in the diseased tissue of Bahamian samples.
  • Control and healthy on diseased microbial community differs with geographical separation.
  • We’re not finished yet!
acknowledgements
Acknowledgements

Special thanks to:

J. Paige Rothenberger

Masoumeh Sikaroodi

Roslyn Cress and the Department of ESP

Perry Institute of Marine Science (PIMS)

National Underwater Research Program (NURP)

American Type Culture Collection (ATCC)

U.S. Fish and Wildlife

The people of St. Croix and the Bahamas

Photo by: Dr. Robert Jonas

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