Streptococcus
This presentation is the property of its rightful owner.
Sponsored Links
1 / 19

STREPTOCOCCUS PowerPoint PPT Presentation


  • 108 Views
  • Uploaded on
  • Presentation posted in: General

STREPTOCOCCUS. 411 , Lab 5 Amany Niazy. Streptococci. + ve. - ve. All are Gram Positive Cocci arranged in chains (short or long) All are Catalase - ve. Gram Stain. Staphylococci. streptococci. Culture. Fastidious organism  no growth on NA Grows well on BAP

Download Presentation

STREPTOCOCCUS

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Streptococcus

STREPTOCOCCUS

411 , Lab 5

AmanyNiazy


Streptococci

Streptococci

+ ve

- ve

All are Gram Positive Cocci arranged in chains (short or long)

All are Catalase -ve


Gram stain

Gram Stain

Staphylococci

streptococci


Culture

Culture

Fastidious organism  no growth on NA

Grows well on BAP

Facultative anaerobe

Grow at 37C need 24 to 48 hour .


Streptococcus classification

Streptococcus Classification

  • Alpha hemolytic

    • S. viridans

    • S. pneumoniae

  • Beta hemolytic:

    • Group A, B, C, G & D

      Lancefield grouping according to antigens in their cell wall

  • Non-hemolytic:

    • Enterococcus (formaly known as Group D


Alpha hemolytic streptococci

Alpha Hemolytic Streptococci

  • Give alpha hemolysis on BAP.

  • It Can be divided in to 2:

    • Viridans group

    • S.pneumoniae


Streptococcus

Streptococcus viridans

s. pneumoniae


Streptococcus

S. PNEUMONIAE

Viridans Streptococci

  • Alpha haemolysis

  • Pin point colonies

  • Optochin resistant

  • Bile Solubility  -ve

  • Part of normal flora of upper respiratory and GI

  • Alpha haemolysis

  • Ring form colonies

  • Tanslucent or mucoid

  • Optochin sensitive

  • Sile solubility  +ve

  • Not part of NF

Not Capsulated

Flattened with raised edges

Capsulated


Streptococcus

Viridans Streptococci

S. PNEUMONIAE

  • GPS in chains

  • GPC elongated (Lanceolated) diplococci


1 optochin test

1. Optochin Test

Always don on a BAP.

On the primary culture of isolation plate before incubation

Ethylhydrocupreine hydrochloride.

Zone of inhibition of 14 mm or more  sensitive S. pneumoniae

No zone of inhibition S.viridans


Optochin disk

Optochin Disk


Bile solubility test

.Bile Solubility Test

  • To test if colonies are lysed by bile salt (such as desoxycholate) or not.

  • There are 2 ways to do the test :

    • In a tube

    • On plate


Bile solubility in tube

Bile Solubility in Tube

  • 2 ml of saline in a tube

  • Emulsify several colonies of test organism in it

  • Divide organism suspension in 2 tubes

  • Add 2 drops of bile salt to one tube and 2 drops of sterile distilled water to the other.

  • Leave 10-25 mn at RT


Bile solubility on plate

Bile Solubility on Plate

  • Add drop of bile salt to specified part of plate

  • Incubate at 37C for 10 - 30 min

  • Colony disappear  +ve

  • Colony stay  -ve


Rapid detection of s pneumoniae

Rapid Detection of S. pneumoniae

Rapid latex and coagglutination tests are available to detect capsular pneumococcal antigen in CSF or other sterile body fluids.


Sensitivity testing of s pneumoniae

Sensitivity Testing of S. pneumoniae


Streptococcus

Thank You


  • Login