Sequential
This presentation is the property of its rightful owner.
Sponsored Links
1 / 12

Sequential genetic changes leading to the evolution of colorectal cancer PowerPoint PPT Presentation


  • 35 Views
  • Uploaded on
  • Presentation posted in: General

Sequential genetic changes leading to the evolution of colorectal cancer. B.Vogelstein: chr5q DNA hypomethylation chr12p chr18q chr17p metastasis FAP loss K-ras DCC loss p53 other alterations.

Download Presentation

Sequential genetic changes leading to the evolution of colorectal cancer

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Sequential genetic changes leading to the evolution of colorectal cancer

Sequential genetic changes leading to the

evolution of colorectal cancer

B.Vogelstein: chr5q DNA hypomethylation chr12p chr18q chr17p metastasis

FAP loss K-ras DCC loss p53 other alterations

chr, chromosome; LOH, loss of heterozygosity

DCC, ‘deleted in colorectal cancer’; FAP, familial polyposis coli


Sequential genetic changes leading to the evolution of colorectal cancer

10-6

106

106

the formation of the tumor cell requires long time

avg. cell cycle time ~ 28-40 hrs


Sequential genetic changes leading to the evolution of colorectal cancer

egér epidermális model: kémiai karcinogenezis -

soklépcsős modell

DMBA(7,12-dimethyl-benzanthracene): Ha-ras-aktiváló mutáció

TPA(12-O-tetradecanoyl-phorbol-13-acetate): cyclin D1 expresszió, papilloma

p53 vesztés: genom destabilizálása

TGF-beta expresszió: szabályozás kiesik, metasztatikus cc. fejlődés

E-cadherin: expresszió csökken, könnyebben mozdulnak el a sejtek


Sequential genetic changes leading to the evolution of colorectal cancer

Accumulation of oncogenic events results in cancer

multistep process

etc.

‘lucky’ situation: loss of altered cell (purple)

cumulation of mis-regulated functions (brown)


Sequential genetic changes leading to the evolution of colorectal cancer

beteg

k-ras

mut

p53ex5

mut

p53ex6

p53ex7

mut

p53ex8

mut

53 F 59

(ctr tum.)

8 gta(V)~gtg(V)

9 gtt(V)~gta(V)

10 gga(G)~gtc(V)

15ggc(G)~ggg(G)

147gtt(V)~gaa(E)

wt

253acc(T)~aac(N)

282 cgg(R)~ctc(L)

283 cgc(R)~cgg(R)

288 aat(N)~aac(N)

289 ctc(L)~cta(L)

291 aag(K)~aac(N)

293ggg(G)~gcc(G)

296 cac(H)~cagQ)

53 F 59

(dist tum.)

12 ggt(G)~gat(D)

wt

wt

249agg(R)~ggg(G)

281 gac(D)~g-c del

CRC beteg, Dukes-B, nagy tumor, két részlet analízise

 König AE 2001

59 é nőbeteg colon cc. centrális és distálsis területéről vett szövetminták mutációs analízise


Sequential genetic changes leading to the evolution of colorectal cancer

Különböző betegekben észlelt mutációs

előfordulások colorectális daganatokban

a stageing szerint

„klasszikus” Vogelstein

genetikai változások emlőrákban és

az EGF-receptor amplifikációja és

kimutathatósága immunhisztokémiával


Sequential genetic changes leading to the evolution of colorectal cancer

a p53 protein szerkezete


Sequential genetic changes leading to the evolution of colorectal cancer

mutációs „hot spot” régiók a p53 proteinben


Sequential genetic changes leading to the evolution of colorectal cancer

Functions of p53 in the various steps of

tumor formation and progression


Sequential genetic changes leading to the evolution of colorectal cancer

theoretical control of carcogenic events


Sequential genetic changes leading to the evolution of colorectal cancer

a daganatsejt és belső-külső környezetének viszonya

környezeti hatások

a sejt válaszai

genetikai változások

a sejt válaszai

hatások a környezetre


  • Login