real world vitamin c concentration and total acidity in orange juice and grapefruit juice
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Real World: Vitamin C Concentration and Total Acidity in Orange Juice and Grapefruit Juice. By: Kate Steinle , Jess Reese, Richard Janosky and Korvin Mercer. Importance of Vitamin C. Cannot be stored in body, needs to be replenished regularly

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real world vitamin c concentration and total acidity in orange juice and grapefruit juice

Real World:Vitamin C Concentration and Total Acidity in Orange Juice and Grapefruit Juice

By: Kate Steinle, Jess Reese, Richard Janosky and Korvin Mercer

importance of vitamin c
Importance of Vitamin C
  • Cannot be stored in body, needs to be replenished regularly
  • Promotes healthy cell development, normal tissue growth and repair, and helps in absorption of calcium.
  • Vitamin C is lost in foods easily, so it is best to eat fruits and vegetables in raw form
  • Scurvy -disease caused by lack of vitamin C
  • It’s an antioxidant, helps protect body against contaminants
measuring the vitamin c content of foods and fruit juices
Measuring the Vitamin C content of foods and fruit juices
    • DCPIP(2,6 – dichlorophenol-indophenol) can be used as an indicator for ascorbic acid.
    • DCPIP turns clear when it is oxidized by the orange juice. When there is excess DCPIP, it no longer turns clear and dyes the sample purple.
  • 1% DCPIP solution added to juice until color change occurred
  • The amount of DCPIP added was recorded
  • Taking into account the amount of vitamin C there should be in the juice based on daily value, the theoretical and actual values of Vitamin C were calculated
measuring the vitamin c content of foods and fruit juices cont d
Measuring the Vitamin C content of foods and fruit juices (cont’d)
  • Theoretical
    • Total volume of container
    • Amount of servings
    • 100% daily value
    • Volume of juice used
  • Experimental
    • 1% DCPIP solution
    • mL of DCPIP added, gives grams of DCPIP
    • 1:1 mol ratio
calculations
Calculations

Theoretical:

Theoretical grams of Vitamin C X Servings= grams vitamin C per bottle

Fresh Squeezed Orange Juice:

0.124g X 2 servings=0.248g vitamin C per bottle

Experimental:

Total volume in bottle/volume added=theoretical grams vitamin C/grams vitamin C in sample

Fresh Squeezed Orange Juice:

473mL/10.31mL=0.248g/x

X=0.0055g

conclusion
Conclusion
  • In all juices, there was almost double the theoretical amount of vitamin C
  • Reason?
    • Companies want to make sure that they have at least the amount of vitamin C in their juice that they claim
    • Too much is not a bad thing
iodine titration
Iodine Titration
  • 25mL of juice samples were titrated with an iodine solution until the endpoint was reached. A 1% starch indicator was used as the indicator
  • The amount of vitamin C was calculated and compared based on daily values for each juice
conclusion1
Conclusion
  • Fresh squeezed orange juice has the highest Vitamin C concentration
  • Fresh squeezed juices have a higher vitamin C concentration than juice from concentrate
determination of the total acidity in orange juice
Determination of the Total Acidity in Orange Juice
  • 3 samples of each juice were titrated with standardized NaOH
  • pH level were measured each addition of NaOH
  • Calibration curve were made
  • pH calculated for each juice
    • Used the curve to estimate equivalence point
    • Another way: change in volume vs. pH
determination of the total acidity in orange juice cont d
Determination of the Total Acidity in Orange Juice (cont’d)
  • Freshly squeezed juices had a higher pH than the juices from concentrate
  • Grapefruit juice more exaggerated relationship
  • Freshly squeezed juices are more acidic
comparing experiments
Comparing Experiments
  • All experiments showed that freshly squeezed juices had more vitamin C
  • Two of three: orange juice had most
bibliography
Bibliography
  • http://www.fitnesshealthzone.com/vitamins-supplements/importance-of-vitamin-c/
  • http://www.practicalbiology.org/areas/advanced/health-and-disease/whats-in-our-food/measuring-the-vitamin-c-content-of-foods-and-fruit-juices,53,EXP.html
  • http://en.wikipedia.org/wiki/Dichlorophenolindophenol
  • http://www.google.com/#sclient=psy&hl=en&q=Determination+of+the+Total+Acidity+in+Orange+Juice&aq=f&aqi=&aql=&oq=&gs_rfai=&pbx=1&fp=fae64975a117d0cd
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