MoL #33472
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pcDNA5 PGK pcDNA5 PGK PowerPoint PPT Presentation


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MoL #33472. shPGK shPGK + dox 0.05ng/ml shPGK + dox 5ng/ml shPGK shPGK + dox 0.05ng/ml shPGK + dox 5ng/ml. Sh-control Sh-control + dox 5ng/ml Sh-control Sh-control + dox 5ng/ml. shHIF-1 α shHIF-1 α + dox 5ng/ml shHIF-1 α shHIF-1 α + dox 5ng/ml. Supplemental Fig.5. pcDNA5 PGK

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pcDNA5 PGK pcDNA5 PGK

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Pcdna5 pgk pcdna5 pgk

MoL #33472

shPGK

shPGK + dox 0.05ng/ml

shPGK + dox 5ng/ml

shPGK

shPGK + dox 0.05ng/ml

shPGK + dox 5ng/ml

Sh-control

Sh-control + dox 5ng/ml

Sh-control

Sh-control + dox 5ng/ml

shHIF-1α

shHIF-1α + dox 5ng/ml

shHIF-1α

shHIF-1α + dox 5ng/ml

Supplemental Fig.5

pcDNA5

PGK

pcDNA5

PGK

Hypoxia - - + + - - + + - - + + - - - + + +

HIF-1α

PGK

APE-1

Supplemental Figure 5. Effect of over-expression of PGK on the levels of L-OddCTP and dFdCTP and their DNA incorporation under normoxic and hypoxic conditions in RKO cells (First column). Effect of induction shHIF-1 using doxcycline on the HIF-1 and PGK expression and the level of L-OddC, dFdCTP and their DNA incorporation under normoxic and hypoxic conditions in RKO cells (Third column). Effect of the induction of shPGK using doxcycline on PGK expression and the levels of L-OddC, dFdCTP and their DNA incorporation under normoxic and hypoxic conditions RKO cells (Fourth column).


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