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Chapter 9: Cellular Respiration. Objectives The student is responsible for: The definitions of all bold faced words in the chapter Knowing the entire chapter. The student is not responsible for: Memorizing or drawing the structures of glycolysis or Kreb’s cycle.

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slide1

Chapter 9: Cellular Respiration

  • Objectives
  • The student is responsible for:
    • The definitions of all bold faced words in the chapter
    • Knowing the entire chapter.
  • The student is not responsible for:
    • Memorizing or drawing the structures of glycolysis or Kreb’s cycle
slide2

Principles of Energy Harvest

Fermentation: decomposition of glucose without the use of oxygen

Cellular Respiration: oxygen is a reactant when glucose is broken down

figure 9 x1 atp
Figure 9.x1 ATP

Adenosine Triphosphate

ATP -> ADP + Pi

ADP  AMP + Pi

figure 9 3 methane combustion as an energy yielding redox reaction
Figure 9.3 Methane combustion as an energy-yielding redox reaction

Oxidizing Agent: that substance that is being reduced.

O is “going” from O (no charge) to O2-.

Reducing Agent: that substance that is being oxidized.

C is gaining oxygen.

figure 9 4 nad as an electron shuttle

The molecule that is used to move hydrogen ions throughout the oxidation of food is NAD+. Therefore NAD+ is an oxidizing agent.

Figure 9.4 NAD+ as an electron shuttle

NAD+ + H+ NADH

figure 9 6 an overview of cellular respiration layer 1
Figure 9.6 An overview of cellular respiration (Layer 1)

But if we could get this pyruvate into the mitochondria we could make a whole lot more ATP!!

figure 9 7 substrate level phosphorylation
Figure 9.7 Substrate-level phosphorylation

What is this substrate-level phosphorylation?

This is when a phosphate group is moved from an organic compound to ADP.

What is oxidative phosphorylation?

When electrons and H+ are used to make ATP.

figure 9 8 the energy input and output of glycolysis
Figure 9.8 The energy input and output of glycolysis

Couldn’t these NADH’s that are made be used to make ATP?

slide19
Figure 9.10 Conversion of pyruvate to acetyl CoA, the junction between glycolysis and the Krebs cycle

Could the NADH produced here be used to make ATP?

figure 9 11 a closer look at the krebs cycle layer 1
Figure 9.11 A closer look at the Krebs cycle (Layer 1)

Keep track of the number of carbons!!

figure 9 13 free energy change during electron transport

NADH and FADH2 deliver electrons to different locations in the ETC.

Figure 9.13 Free-energy change during electron transport

The role of oxygen is to serve as a hydrogen ion acceptor to form water.

figure 9 14 atp synthase a molecular mill

ATP Synthase

Chemiosmosis: the coupling of the movement of H+ through a protein complex (ATP Synthase) making ATP.

Figure 9.14 ATP synthase, a molecular mill
figure 9 15 chemiosmosis couples the electron transport chain to atp synthesis
Figure 9.15 Chemiosmosis couples the electron transport chain to ATP synthesis

A Proton-Motive Force is produced

slide28
Figure 9.16 Review: how each molecule of glucose yields many ATP molecules during cellular respiration
slide33

Versatility of Catabolism

  • Use of Proteins
    • Proteins  amino acids and then the amino acids must have their amino groups removed before being used as an energy source. So all the energy bars that have amino acids in them are at least one step closer to being used for energy than a protein.
    • Fats must go through beta oxidation which takes a fat and breaks off 2 carbon fragments from the fatty acids and these 2 carbon fragments enter at acetyl-CoA.
figure 9 20 the control of cellular respiration
Figure 9.20 The control of cellular respiration 
  • The Control of Cellular Respiration
  • PFK: allosteric enzyme
    • Receptor sites for ATP, AMP and citrate
    • ATP: inhibitor
    • AMP: stimulator
    • Citrate: inhibitor
slide35

The Evolutionary Significance of Glycolysis

Earliest organisms were in an anaerobic environment (3.5 billion yrs ago)

Glycolysis was probably used as an energy making process

Oxygen accumulated about 2.7 billion years ago

Glycolysis is the most widespread pathway amongst organisms suggesting it evolved early on.

Glycolysis requires only the cytoplasm and membrane-bound organelles were not present until eukaryotic cells appeared (2 billion years after prokaryotes)

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