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Vimentin. FLAG/Luc/#1. FLAG/Keap1/#1. FLAG/Luc/#1 FLAG/Keap1/#1. B. Supplemental Figure 1. A. Supplemental Figure 2. GSH. 25,000,000. 20,000,000. Relative Intensity. 15,000,000. 10,000,000. 5,000,000. 0. Control. Nrf2 shRNA/#2. Levels of ROS. A. B. Control.

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Supplemental figure 1

Vimentin

FLAG/Luc/#1

FLAG/Keap1/#1

FLAG/Luc/#1 FLAG/Keap1/#1

B

Supplemental Figure 1

A


Supplemental figure 1

Supplemental Figure 2

GSH

25,000,000

20,000,000

Relative Intensity

15,000,000

10,000,000

5,000,000

0

Control

Nrf2 shRNA/#2

Levels of ROS

A

B

Control

Nrf2 shRNA

Cell Number

C400

Control shRNA

Nrf2 shRNA/#2

C

Number x 10


Supplemental figure 1

Nrf2 shRNA #2

Control shRNA

pAKT

AKT

C-Jun

Cyclin D1

GAPDH

Supplemental Figure 3


Supplemental figure 1

A549 cells

Control lentivirus shRNA

Lentivirus Nrf2 shRNA clone 3

Lentivirus Nrf2 shRNA clone 6

Nrf2

Cell Number

GAPDH

Control

Clone 3

Days

Lentivirus Nrf2 shRNA

clones

Supplemental Figure 4

B

Clone 6


Supplemental figure 1

Supplemental Figure 5

Nrf2 shRNA

Control shRNA


Supplemental figure 1

0 hr 24 hr 24 hr

5 ng/ml TGF- SB431532 + TGF-

Supplemental Figure 6

B

A

Control retrovirus

shRNA

Nrf2 siRNA in pSilencer

Retrovirus Nrf2 shRNA #2

Control siRNA in pSilencer

O hr 24hr

24 hrs

C


Supplemental figure 1

24 hrs + TGF-

24 hrs TGF- + SIS3

Supplemental Figure 7


Supplemental figure 8

Supplemental Figure 8

Micro-Taur schematic diagram

“A” side media ports

Cell loading port

“B” side media ports

  • 6 micron posts

  • 8 micron posts

  • 10 micron posts

  • 12 micron posts

Post-containing

chambers

Insert illustrating Post Region (12 micron)


Supplemental figure 1

Supplemental Figure 9

2500

2000

1500

TGF-1 pg/ml

1000

500

0

Control shRNA

Nrf2-shRNA #2


Supplemental figure 1

A

B

Loss of Nrf2 expression

TGF β

TGF β

E-Cadherin

ROS

ROS

Phosphatases

P P

P P

P P

Phosphatases

PP

PP

PP

PP

MH2

MH2

MH2

MH2

MH1

MH1

MH1

MH1

MH2

MH1

R-Smad

R-Smad

R-Smad

R-Smad

R-Smad

pR-Smad

pR-Smad

pR-Smad

Smad4

Smad4

Smad4

Nrf2

Smad target genes

Smad target genes

Nrf2

Nrf2

Slug

Slug

Smad target genes

Supplemental Figure 10

E-Cadherin


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