The effect of resveratrol on fat storage in c elegans
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The Effect of Resveratrol on Fat Storage in C. elegans. By Chris LoBello. Obesity. http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps. Adipocytes. http://whyfiles.org/276metabolic_syndrome/index.php?g=3.txt. http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg. PPAR g.

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Obesity
Obesity

http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps


Adipocytes

http://whyfiles.org/276metabolic_syndrome/index.php?g=3.txt


http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpghttp://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg


PPARhttp://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpgg

http://www.juliantrubin.com/encyclopedia/biochemistry/ppar.html


Chung and kurtev 2004
Chung and Kurtev (2004)http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg

http://eresveratrol.com/content/00/01/43/84/95/userimages/ppar_gamma.pdf


Wood and Sinclair (2004)http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg

Fei and Liu (2004)

http://www.nature.com/nature/journal/v430/n7000/fig_tab/nature02789_F2.html

http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1224044&blobtype=pdf


Methodology

Control Group: 0 microliters of resveratrolhttp://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg

N= N2 Wild Type Strain Culture

Group 1: 1 microliter of resveratrol

N= N2 Wild Type Strain Culture

Group 2: 10 microliters of resveratrol

N= N2 Wild Type Strain Culture

Group 3: 50 microliters of resveratrol

N= N2 Wild Type Strain Culture

Group 4: 100 microliters of resveratrol

N= N2 Wild Type Strain Culture

Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol

Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol

RT-PCR to determine if SIRT1 is activated

Quantum yield of fluorescent intensity determined using SensL photon counter (counts per nanosecond)

Statistical Analysis by ANOVA where p <.05 followed by Scheffe post hoc test

Methodology

The Effect of Resveratrol on Fat Storage in C. elegans


Budget
Budgethttp://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg


Purpose
Purposehttp://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg

To determine the effects of varying amounts of resveratrol on the fat content of C. elegans


Hypothesis
Hypothesishttp://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg

Null Hypothesis: There will be equal amounts of fat content in cells with resveratrol supplemented and without resveratrol supplemented

Alternate Hypothesis: There will be less fat content in the cells supplemented with resveratrol, and that there will be an inverse relationship between resveratrol supplementation and fat content


Bibliography
Bibliographyhttp://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg

  • "Peroxisome Proliferator-Activated Receptors (PPARs) for Science Fair Projects." The Orchid Grower: A Juvenile Science Adventure Novel. 15 May 2009 <http://www.juliantrubin.com/encyclopedia/biochemistry/ppar.html>.

  • Golden, T.R. and Melov, S. Gene expression changes associated with aging in C. elegans (January 2, 2007), WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.127.1,http://www.wormbook.org

  • Pcard, Frederic, Martin Kurtev, Namjin Chung, Acharawan Topark-Ngarm, Thanaset Senawong, Rita Machado de Oliveira, Mark Leid, Michael W. McBurney, and Leonard Guarente. "Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-g." Nature (2004).

  • Yamamoto, Hiroyasu, Kristina Schoonjans, and Johan Auwerx. "Sirtuin Functions in Health and Disease." (2007). Minireview. Molecular Endocrinology. 5 May 2009 <http://mend.endojournals.org/cgi/content/full/21/8/1745>.

  • Longo, Valter D., and Brian K. Kennedy. "Sirtuins in Aging and Age-Related Disease." (2006). Leading Edge Review. Cell. 5 May 2009 <http://beadle.rutgers.edu/MBB/101/101-08/101-Papers/Sir2-review.pdf>.

  • "PPAR Resource Page." Home Page | PPAR Resource Page | Jack VandenHeuvel. 05 May 2009 <http://ppar.cas.psu.edu/pparrfront.htm >.

  • "PPARG Gene - GeneCards | PPARG Protein | PPARG Antibody." Human Genes | Gene Database | Gene Search - GeneCards. 05 May 2009 < http://www.genecards.org/cgi-bin/carddisp.pl?gene=PPARg&ortholog=all#ort>.

  • "Search Our Gene Guide Database – Find Products by Gene Category, Human Gene Category or Species." IGene. 15 May 2009 <http://igene.invitrogen.com/igene/home.do>.

  • "Obesity and Overweight: Trends: U.S. Obesity Trends | DNPAO | CDC." Centers for Disease Control and Prevention. 17 May 2009 <http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps>.

  • Frye, Roy A. "Phylogenetic Classification of Prokaryotic and Eukaryotic Sir2-like Proteins." ScienceDirect (2000). 31 May 2000. Pittsburgh V.A. Medical Center. 18 May 2009 <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-45FKB8B

  • "Main Posterboard on Gene-Quantification.info." Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform. 18 May 2009 <http://www.gene-quantification.de/poster-main.html>.

  • "Worm Procreation: 50 Ways to Be Your Lover ::." University Communications Newsdesk, University of Maryland. 18 May 2009 <http://www.newsdesk.umd.edu/scitech/release.cfm?ArticleID=1242>.

  • "Peroxisome Proliferator-Activated Receptors (PPARs) for Science Fair Projects." The Orchid Grower: A Juvenile Science Adventure Novel. 18 May 2009 <http://www.juliantrubin.com/encyclopedia/biochemistry/ppar.html>.

  • "The Why Files | 3. Chewing the fat on metabolic syndrome." The Why Files | The Science Behind the News. 18 May 2009 <http://whyfiles.org/276metabolic_syndrome/index.php?g=3.txt>.

  • Nature Publishing Group : science journals, jobs, and information. 19 May 2009 <http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg>.

  • Wolf, George. "Calorie Restriction Increases Life Span: A Molecular Mechanism." Wiley InterScience (2006). Nutrition Reviews. <http://www3.interscience.wiley.com/journal/119819972/abstract>.

  • Tissenbaum, Heidi A., and Leonard Guarente. "Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans." Nature (2001). Letters to Nature. 2 Jan. 2001. Department of Biology, Massachusetts Institute of Technology. 19 May 2009 <http://www.nature.com/nature/journal/v410/n6825/abs/410227a0.html>.

  • Shi, Tong, Fei Wang, Qiang Tong, and Emily Stieren. "SIRT3, a Mitochondrial Sirtuin Deacetylase, Regulates Mitochondrial Function and Thermogenesis in Brown Adipocytes." JBC online (2005). 8 Apr. 2005. Baylor College of Medicine. 19 May 2009 <http://www.jbc.org/cgi/content/full/280/14/13560>.

  • "The Skinny on Fat." The Interactive Media Lab at the University of Florida. 20 May 2009 <http://iml.jou.ufl.edu/projects/Fall05/holman/statistics.html>.

  • "Resveratrol Molecule --Polyphenol." World of Molecules Home Page. 21 May 2009 <http://www.worldofmolecules.com/antioxidants/resveratrol.htm>.

  • “AMERICA AND ADIPOCYTES: WHY LIPOSUCTION IS NOT THE ANSWER." The Science Creative Quarterly. 21 May 2009 <http://www.scq.ubc.ca/america-and-adipocytes-why-liposuction-is-not-the-answer/>.

  • Fei, You-Jun, Jin-Cai Liu, Katsuhisa INOUE, LinaZhuang, Katsuya MIYAKE, Seiji MIYAUCHI, and Vadivel GANAPATHY. "Relevance of NAC-2, an Na+-coupled citrate transporter, to life span, body." Biochemical Study (2004). 2004. Department of Biochemistry and Molecular Biology, Medical College of Georgia. 21 May 2009 <http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1224044&blobtype=pdf>.

  • Kumanyika, Shiriki K., Youfa Wang, May A. Beydoun, Lan Liang, and Benjamin Caballero. "Will All Americans Become Overweight or Obese? Estimating the Progression and Cost of the US Obesity Epidemic." Obesity: A Research Journal (2008). Epidemiology. 24 July 2008. University of Pennsylvania School of Medicine. 21 May 2009 <http://www.nature.com/oby/journal/v16/n10/full/oby2008351a.html>.

  • Wood, Mason G., Konrad Howitz, Steven L. Helfand, Marc Tatar, David Sinclair, BlankaRogina, and Siva Lavu. Sirtuin activators mimic caloric restriction and delay ageing in metazoans (2004). 5 Aug. 2004. 25 May 2009 http://www.nature.com/nature/journal/v430/n7000/fig_tab/nature02789_F2.html

  • "Introduction to C. Elegans." Genes, Genomes, and Human Genetics. 26 May 2009 <http://avery.rutgers.edu/WSSP/StudentScholars/project/introduction/worms.html>.

  • Stiernagle, T. Maintenance of (February 11, 2006), , ed. The Research Community, WormBook, doi/10.1895/wormbook.1.101.1, http://www.wormbook.org.

  • Epstein, Henry F., and Diane C. Shakes. Methods in Cell Biology. Vol. 48. Academic P, 1995. Modern Biological Analysis of an Organism. 1995. 28 May 2009 <http://books.google.com/books?hl=en&lr=&id=AUxd_e4mzHIC&oi=fnd&pg=PA3&dq=Basic+culture+methods&ots=ViJ4m8r4zY&sig=mRx6Zc5HuXIFEOvpcq0sA4eG89k#PPP1,M1>.

  • Sklar, Larry A., ed. Flow Cytometry for Biotechnology. Oxford UP. Google Book. Oxford. 1 June 2009 <http://books.google.com/books?id=IoNFY2D2_tMC&pg=PA16&lpg=PA16&dq=cytometry+fluorescence&source=bl&ots=RjO_jgeXGh&sig=6yhmU8lz4nADX-KabZHvmyP-FZM&hl=en&ei=SWAjSoPQMsG-tweIqqWvBg&sa=X&oi=book_result&ct=result&resnum=7#PPA15,M1>.

  • "Flow Cytometry Principles." Biology @ Berkeley. 01 June 2009 <http://biology.berkeley.edu/crl/flow_cytometry_basic.html>.

  • Bellis, Stephen, Andreas Konig, and Carl Jackson. "Photon Counting Sensors for Medical and Biophotonic Applications." Laser+photonik (2005). PECIAL: BIOPHOTONICS Optical Detectors. 2005. 2 June 2009 <http://sensl.com/pdfs/Laser_Photnik_Facatikel_Photonen_English.pdf>.


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