the effect of resveratrol on fat storage in c elegans
Download
Skip this Video
Download Presentation
The Effect of Resveratrol on Fat Storage in C. elegans

Loading in 2 Seconds...

play fullscreen
1 / 12

The Effect of Resveratrol on Fat Storage in C. elegans - PowerPoint PPT Presentation


  • 64 Views
  • Uploaded on

The Effect of Resveratrol on Fat Storage in C. elegans. By Chris LoBello. Obesity. http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps. Adipocytes. http://whyfiles.org/276metabolic_syndrome/index.php?g=3.txt. http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg. PPAR g.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' The Effect of Resveratrol on Fat Storage in C. elegans ' - shafira-ramos


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
obesity
Obesity

http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps

slide3

Adipocytes

http://whyfiles.org/276metabolic_syndrome/index.php?g=3.txt

slide4

http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpghttp://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg

slide5

PPARg

http://www.juliantrubin.com/encyclopedia/biochemistry/ppar.html

chung and kurtev 2004
Chung and Kurtev (2004)

http://eresveratrol.com/content/00/01/43/84/95/userimages/ppar_gamma.pdf

slide7

Wood and Sinclair (2004)

Fei and Liu (2004)

http://www.nature.com/nature/journal/v430/n7000/fig_tab/nature02789_F2.html

http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1224044&blobtype=pdf

methodology

Control Group: 0 microliters of resveratrol

N= N2 Wild Type Strain Culture

Group 1: 1 microliter of resveratrol

N= N2 Wild Type Strain Culture

Group 2: 10 microliters of resveratrol

N= N2 Wild Type Strain Culture

Group 3: 50 microliters of resveratrol

N= N2 Wild Type Strain Culture

Group 4: 100 microliters of resveratrol

N= N2 Wild Type Strain Culture

Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol

Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol

RT-PCR to determine if SIRT1 is activated

Quantum yield of fluorescent intensity determined using SensL photon counter (counts per nanosecond)

Statistical Analysis by ANOVA where p <.05 followed by Scheffe post hoc test

Methodology

The Effect of Resveratrol on Fat Storage in C. elegans

purpose
Purpose

To determine the effects of varying amounts of resveratrol on the fat content of C. elegans

hypothesis
Hypothesis

Null Hypothesis: There will be equal amounts of fat content in cells with resveratrol supplemented and without resveratrol supplemented

Alternate Hypothesis: There will be less fat content in the cells supplemented with resveratrol, and that there will be an inverse relationship between resveratrol supplementation and fat content

bibliography
Bibliography
  • "Peroxisome Proliferator-Activated Receptors (PPARs) for Science Fair Projects." The Orchid Grower: A Juvenile Science Adventure Novel. 15 May 2009 <http://www.juliantrubin.com/encyclopedia/biochemistry/ppar.html>.
  • Golden, T.R. and Melov, S. Gene expression changes associated with aging in C. elegans (January 2, 2007), WormBook, ed. The C. elegans Research Community, WormBook, doi/10.1895/wormbook.1.127.1,http://www.wormbook.org
  • Pcard, Frederic, Martin Kurtev, Namjin Chung, Acharawan Topark-Ngarm, Thanaset Senawong, Rita Machado de Oliveira, Mark Leid, Michael W. McBurney, and Leonard Guarente. "Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-g." Nature (2004).
  • Yamamoto, Hiroyasu, Kristina Schoonjans, and Johan Auwerx. "Sirtuin Functions in Health and Disease." (2007). Minireview. Molecular Endocrinology. 5 May 2009 <http://mend.endojournals.org/cgi/content/full/21/8/1745>.
  • Longo, Valter D., and Brian K. Kennedy. "Sirtuins in Aging and Age-Related Disease." (2006). Leading Edge Review. Cell. 5 May 2009 <http://beadle.rutgers.edu/MBB/101/101-08/101-Papers/Sir2-review.pdf>.
  • "PPAR Resource Page." Home Page | PPAR Resource Page | Jack VandenHeuvel. 05 May 2009 <http://ppar.cas.psu.edu/pparrfront.htm >.
  • "PPARG Gene - GeneCards | PPARG Protein | PPARG Antibody." Human Genes | Gene Database | Gene Search - GeneCards. 05 May 2009 < http://www.genecards.org/cgi-bin/carddisp.pl?gene=PPARg&ortholog=all#ort>.
  • "Search Our Gene Guide Database – Find Products by Gene Category, Human Gene Category or Species." IGene. 15 May 2009 <http://igene.invitrogen.com/igene/home.do>.
  • "Obesity and Overweight: Trends: U.S. Obesity Trends | DNPAO | CDC." Centers for Disease Control and Prevention. 17 May 2009 <http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps>.
  • Frye, Roy A. "Phylogenetic Classification of Prokaryotic and Eukaryotic Sir2-like Proteins." ScienceDirect (2000). 31 May 2000. Pittsburgh V.A. Medical Center. 18 May 2009 <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-45FKB8B
  • "Main Posterboard on Gene-Quantification.info." Gene-Quantification.info - The reference in qPCR - Academic & Industrial Information Platform. 18 May 2009 <http://www.gene-quantification.de/poster-main.html>.
  • "Worm Procreation: 50 Ways to Be Your Lover ::." University Communications Newsdesk, University of Maryland. 18 May 2009 <http://www.newsdesk.umd.edu/scitech/release.cfm?ArticleID=1242>.
  • "Peroxisome Proliferator-Activated Receptors (PPARs) for Science Fair Projects." The Orchid Grower: A Juvenile Science Adventure Novel. 18 May 2009 <http://www.juliantrubin.com/encyclopedia/biochemistry/ppar.html>.
  • "The Why Files | 3. Chewing the fat on metabolic syndrome." The Why Files | The Science Behind the News. 18 May 2009 <http://whyfiles.org/276metabolic_syndrome/index.php?g=3.txt>.
  • Nature Publishing Group : science journals, jobs, and information. 19 May 2009 <http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg>.
  • Wolf, George. "Calorie Restriction Increases Life Span: A Molecular Mechanism." Wiley InterScience (2006). Nutrition Reviews. <http://www3.interscience.wiley.com/journal/119819972/abstract>.
  • Tissenbaum, Heidi A., and Leonard Guarente. "Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans." Nature (2001). Letters to Nature. 2 Jan. 2001. Department of Biology, Massachusetts Institute of Technology. 19 May 2009 <http://www.nature.com/nature/journal/v410/n6825/abs/410227a0.html>.
  • Shi, Tong, Fei Wang, Qiang Tong, and Emily Stieren. "SIRT3, a Mitochondrial Sirtuin Deacetylase, Regulates Mitochondrial Function and Thermogenesis in Brown Adipocytes." JBC online (2005). 8 Apr. 2005. Baylor College of Medicine. 19 May 2009 <http://www.jbc.org/cgi/content/full/280/14/13560>.
  • "The Skinny on Fat." The Interactive Media Lab at the University of Florida. 20 May 2009 <http://iml.jou.ufl.edu/projects/Fall05/holman/statistics.html>.
  • "Resveratrol Molecule --Polyphenol." World of Molecules Home Page. 21 May 2009 <http://www.worldofmolecules.com/antioxidants/resveratrol.htm>.
  • “AMERICA AND ADIPOCYTES: WHY LIPOSUCTION IS NOT THE ANSWER." The Science Creative Quarterly. 21 May 2009 <http://www.scq.ubc.ca/america-and-adipocytes-why-liposuction-is-not-the-answer/>.
  • Fei, You-Jun, Jin-Cai Liu, Katsuhisa INOUE, LinaZhuang, Katsuya MIYAKE, Seiji MIYAUCHI, and Vadivel GANAPATHY. "Relevance of NAC-2, an Na+-coupled citrate transporter, to life span, body." Biochemical Study (2004). 2004. Department of Biochemistry and Molecular Biology, Medical College of Georgia. 21 May 2009 <http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1224044&blobtype=pdf>.
  • Kumanyika, Shiriki K., Youfa Wang, May A. Beydoun, Lan Liang, and Benjamin Caballero. "Will All Americans Become Overweight or Obese? Estimating the Progression and Cost of the US Obesity Epidemic." Obesity: A Research Journal (2008). Epidemiology. 24 July 2008. University of Pennsylvania School of Medicine. 21 May 2009 <http://www.nature.com/oby/journal/v16/n10/full/oby2008351a.html>.
  • Wood, Mason G., Konrad Howitz, Steven L. Helfand, Marc Tatar, David Sinclair, BlankaRogina, and Siva Lavu. Sirtuin activators mimic caloric restriction and delay ageing in metazoans (2004). 5 Aug. 2004. 25 May 2009 http://www.nature.com/nature/journal/v430/n7000/fig_tab/nature02789_F2.html
  • "Introduction to C. Elegans." Genes, Genomes, and Human Genetics. 26 May 2009 <http://avery.rutgers.edu/WSSP/StudentScholars/project/introduction/worms.html>.
  • Stiernagle, T. Maintenance of (February 11, 2006), , ed. The Research Community, WormBook, doi/10.1895/wormbook.1.101.1, http://www.wormbook.org.
  • Epstein, Henry F., and Diane C. Shakes. Methods in Cell Biology. Vol. 48. Academic P, 1995. Modern Biological Analysis of an Organism. 1995. 28 May 2009 <http://books.google.com/books?hl=en&lr=&id=AUxd_e4mzHIC&oi=fnd&pg=PA3&dq=Basic+culture+methods&ots=ViJ4m8r4zY&sig=mRx6Zc5HuXIFEOvpcq0sA4eG89k#PPP1,M1>.
  • Sklar, Larry A., ed. Flow Cytometry for Biotechnology. Oxford UP. Google Book. Oxford. 1 June 2009 <http://books.google.com/books?id=IoNFY2D2_tMC&pg=PA16&lpg=PA16&dq=cytometry+fluorescence&source=bl&ots=RjO_jgeXGh&sig=6yhmU8lz4nADX-KabZHvmyP-FZM&hl=en&ei=SWAjSoPQMsG-tweIqqWvBg&sa=X&oi=book_result&ct=result&resnum=7#PPA15,M1>.
  • "Flow Cytometry Principles." Biology @ Berkeley. 01 June 2009 <http://biology.berkeley.edu/crl/flow_cytometry_basic.html>.
  • Bellis, Stephen, Andreas Konig, and Carl Jackson. "Photon Counting Sensors for Medical and Biophotonic Applications." Laser+photonik (2005). PECIAL: BIOPHOTONICS Optical Detectors. 2005. 2 June 2009 <http://sensl.com/pdfs/Laser_Photnik_Facatikel_Photonen_English.pdf>.
ad