USES OF ENGINEERED PROTEINS ADVANDAGES OF PROTEIN ENGINEERING BIOETHICS. BY K.PANDI SELVI II.M.SC.,MARINE BIOTECHNOLOGY. Proteins are polymeric molecules constructed from twenty building blocks called aminoacids. Proteins have diverse functions.
ADVANDAGES OF PROTEIN ENGINEERING
Recombinant DNA techniques in E.coli:-
The expression of heterologous genes in bacteria is the simplest means for obtaining large amounts of desired proteins.
Due to availability of promoters there is efficient production of recombinant proteins.
E.colican readily to grown to high cell density.
This helps in the production of therapeutic & industrial proteins on a large scale.
E.coli, gram negative bacteria contains two membrane.
It is divided into three compartments:
The mode of gene expression affects the location of protein produced.
High cell density culture techniques for E.colihave been developed to improved productivity.
Many proteins expressed in E.coli were documented intracellularly & biological activity must be recovered.
Advances in protein refolding, translocation & roles of molecular chaperons & foldases have made possible the design of recombinant E.coli strains.
They strengthen the dominant status of E.coli as a host for production of recombinant protein.
Advantages and Disadvantages in the protein production into each compartment of Escherichia coli
Diagram describing the function of the lac repressor (LacI). be applied only to proteins for which a reliable homology model can be obtained.
Fig: Western blot analysis of protein expression be applied only to proteins for which a reliable homology model can be obtained..
It is a philosophical study of ethical controversies brought about by advances in biology & medicine, life sciences, biotechnology, politics, law & philosophy.
To encourage the beneficial applications of this powerful technology.
Also, limiting its potential for misuse.
The appropriate review & oversight mechanisms should be established.
: established by
Sandhya mitra.,Genetic engineering principles &
Practice., First edition (1996)., Macmillon limited, Delhi.
Helen kreuzer.,Adrianne massey., Recombinant DNA &
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