USES OF ENGINEERED PROTEINS ADVANDAGES OF PROTEIN ENGINEERING BIOETHICS. BY K.PANDI SELVI II.M.SC.,MARINE BIOTECHNOLOGY. Proteins are polymeric molecules constructed from twenty building blocks called aminoacids. Proteins have diverse functions.
USES OF ENGINEERED PROTEINS
ADVANDAGES OF PROTEIN ENGINEERING
What is proteinengineering?
Recombinant DNA techniques in E.coli:-
The expression of heterologous genes in bacteria is the simplest means for obtaining large amounts of desired proteins.
Due to availability of promoters there is efficient production of recombinant proteins.
E.colican readily to grown to high cell density.
This helps in the production of therapeutic & industrial proteins on a large scale.
E.coli, gram negative bacteria contains two membrane.
It is divided into three compartments:
The mode of gene expression affects the location of protein produced.
High cell density culture techniques for E.colihave been developed to improved productivity.
Many proteins expressed in E.coli were documented intracellularly & biological activity must be recovered.
Advances in protein refolding, translocation & roles of molecular chaperons & foldases have made possible the design of recombinant E.coli strains.
They strengthen the dominant status of E.coli as a host for production of recombinant protein.
Advantages and Disadvantages in the protein production into each compartment of Escherichia coli
Advantages & Disadvantages
Use of Engineered proteins for Organization of Nanostructure
Diagram describing the function of the lac repressor (LacI).
Characterization of lac I-338::QBP3
Fig: Western blot analysis of protein expression.
RESULTS & CONCLUSION
It is a philosophical study of ethical controversies brought about by advances in biology & medicine, life sciences, biotechnology, politics, law & philosophy.
Bioethics in protein engineering
To encourage the beneficial applications of this powerful technology.
Also, limiting its potential for misuse.
The appropriate review & oversight mechanisms should be established.
Sandhya mitra.,Genetic engineering principles &
Practice., First edition (1996)., Macmillon limited, Delhi.
Helen kreuzer.,Adrianne massey., Recombinant DNA &