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Pyruvate Oxidation Pyruvate dehydrogenase complex

Pyruvate Oxidation Pyruvate dehydrogenase complex. Aerobic Fate of Pyruvate: Overview. Mitochondria: Site of Oxidations. Pyruvate Oxidation to Acetyl-CoA. Pyruvate Dehydrogenase Complex. Complex enzyme 60 polypeptides of 3 kinds In mitocondrion matrix Regulated Inhibited by NADH and GTP

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Pyruvate Oxidation Pyruvate dehydrogenase complex

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  1. Pyruvate OxidationPyruvate dehydrogenase complex

  2. Aerobic Fate of Pyruvate: Overview

  3. Mitochondria: Site of Oxidations

  4. Pyruvate Oxidation to Acetyl-CoA

  5. Pyruvate Dehydrogenase Complex • Complex enzyme • 60 polypeptides of 3 kinds • In mitocondrion matrix • Regulated • Inhibited by NADH and GTP • Stimulated by insulin

  6. Pyruvate Dehydrogenase Complex • Three enzymes and 5 coenzymes Total MW: 4.5 x 106! Also present: regulatory protein kinase, phosphatase

  7. Lipoic Acid: Acyl- and Redox Carrier

  8. Thiamine Pyrophosphate (TPP)

  9. Pyruvate Dehydrogenase Mechanism

  10. Pyruvate Dehydrogenase Mechanism

  11. Pyruvate Dehydrogenase Mechanism

  12. Pyruvate Dehydrogenase Mechanism

  13. Pyruvate Dehydrogenase Mechanism

  14. Pyruvate Dehydrogenase Mechanism

  15. Pyruvate Dehydrogenase Mechanism

  16. Pyruvate Dehydrogenase Mechanism

  17. PDH: The Overall Reaction • Acetyl-CoA enter Krebs cycle • NADH passes e– to O2

  18. -Ketoglutarate Dehydrogenase Complex ∆Gº= –33.5 kJ/mol

  19. -Ketoglutarate Dehydrogenase Complex • Cofactors: FAD, NAD+, lipoate, TPP, CoASH • Mechanism  PDH complex

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