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Role of CD4 T-Cell Help in Activating Memory CD8 T-Cells Against Tumors

This study investigates the role of CD4 T-helper cells in activating memory CD8 T-cells to function as effective tumor killers. It also examines the efficacy of antigen-specific CD4 T-cell help in tumor protection.

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Role of CD4 T-Cell Help in Activating Memory CD8 T-Cells Against Tumors

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  1. Antigen-specific CD4T-Cell Help Is Required to Activate a Memory CD8T Cellto a Fully Functional Tumor Killer Cell 高丰光

  2. Background • CD8 mCTLs3 play a major role in the elimination of viruses and tumors • Before mCTLs can eliminate viruses and tumors, these cells are required to roliferate and acquire effector function. • The role CD4 T-helper cells play in the induction of CD8 effector T cells from naive precursors is known • the role of T-helper cells in the activation of mCTLs when the antigen is presented by the tumor is not established.

  3. Question • How about T-helper cells in the activation of memory T cells?

  4. 相关说明 • OT-1 mice : 表达SIINFEKL特异性TCR • OVA：鸡卵白蛋白 • OVA CTL epitope: SIINFEKL • OVA Th epitope:ISQAVHAAHAEI-NEAGROVA （OVT） • RAG-1 KO mice: • C57BL/6 mice: • IFA/CFA/KLH • EG7 cell line • EL4 cell line

  5. 相关步骤 • mCTL and eCTL generation • specific Th generation • mCTL transfer • tumor challenge

  6. OT-1 mice 的基本特征

  7. mCTL generation

  8. mCTL generation

  9. mCTL generation

  10. CD8+Memory T Cells Need Antigen-specific CD4+T-helper Cells to Achieve Tumor Protection

  11. Absence of tumor protection in mice without antigen-specific T-helper cells is not because of lower levels of tumor antigen (SIINFEKL)-specific CD8+T cells.

  12. In contrast to mCTLs, eCTLs do not need T help to kill tumor

  13. Study of the long-lived memory CD8+T cells generated in C57BL/6J mice

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