'Transformation solution' presentation slideshows

Biotechnology

Biotechnology. Explorer Program. Serious About Science Education. Aequorea victoria. DNA. RNA. Protein. Trait. Central Framework of Molecular Biology. Transformation is a natural process that Bacterial have evolved in order to obtain DNA from their environment.

388 views • 29 slides

Way to Glow! Bacterial Transformation

Way to Glow! Bacterial Transformation. Transformation of E. coli with pFluoroGreen. What is our goal?. To express green fluorescent protein ( GFP) into transformed bacterial cells. Laboratory Safety. Gloves Goggles Lab coat Non-pathogenic strain of E. coli. Transformation of E. coli.

689 views • 28 slides

670 views • 25 slides

Transformation of E. coli with Green Fluorescent Protein (GFP)

Transformation of E. coli with Green Fluorescent Protein (GFP). DNA. RNA. Protein. Trait. Central Framework of Molecular Biology. Links to Real-world. GFP is a visual marker Study of biological processes (example: synthesis of proteins)

253 views • 23 slides

Genetic Engineering

357 views • 19 slides

pGLO TM Bacterial Transformation Courtesy BioRad Corporation

pGLO TM Bacterial Transformation Courtesy BioRad Corporation. pGLO/ Green Fluorescent Protein Isolation A single bacteria might be worth 5 billion dollars to the right buyer. DNA. RNA. Protein. Trait. Central Framework of Molecular Biology. What is transformation?.

642 views • 18 slides

Transformation Procedure Overview

Transformation Procedure Overview. Day 2. Day 1. What is Transformation?. Uptake of foreign DNA, often a circular plasmid. GFP. Beta-lactamase Ampicillin Resistance. Bacterial DNA. Bacterial cell. Plasmid DNA. Genomic DNA. The Many Faces of Plasmids. Graphic representation.

306 views • 16 slides

Transformation Procedure Summary Incorporation of Foreign DNA Adhere DNA to Cells on ice Heat shock and DNA Entry Recove

Transformation Procedure Summary Incorporation of Foreign DNA Adhere DNA to Cells on ice Heat shock and DNA Entry Recovery on ice Plating Cells Screen for Transformants. 10 ul of pGlo (loopful of plasmid DNA) + one colony. 250 ul CaCl 2 (Transformation Solution) on ice for 10 min.

301 views • 10 slides