'Transformation efficiency' presentation slideshows

Chlorop l ast T rans f ormat i on and its Applications to Plant Improvement

Chlorop l ast T rans f ormat i on and its Applications to Plant Improvement. What is Chloroplast ?.

442 views • 44 slides

Way to Glow! Bacterial Transformation

Way to Glow! Bacterial Transformation. Transformation of E. coli with pFluoroGreen. What is our goal?. To express green fluorescent protein ( GFP) into transformed bacterial cells. Laboratory Safety. Gloves Goggles Lab coat Non-pathogenic strain of E. coli. Transformation of E. coli.

688 views • 28 slides

634 views • 24 slides

Analyzing Data

Analyzing Data. Transformation. The DNA molecule is hydrophilic (water-soluble) but cell membranes are made of a very hydrophobic lipid bilayer. Two means of artificial transformation commonly used in labs: electroporation and chemical transformation.

519 views • 22 slides

Analyzing Data

Analyzing Data. Transformation. The DNA molecule is hydrophilic (water-soluble) but cell membranes are made of a very hydrophobic lipid bilayer. Two means of artificial transformation commonly used in labs: electroporation and chemical transformation.

561 views • 22 slides

Bacterial Transformation

Bacterial Transformation. Broad and Long Term Objective. To characterize a single clone from an Emiliania huxleyi cDNA library using sequence analysis. Research Plan. Preparation of Competent Cells and Bacterial Transformation. Growth of Transformant and Plasmid MiniPrep.

545 views • 20 slides

pGLO TM Bacterial Transformation

pGLO TM Bacterial Transformation. Extension Activity: Calculation of Transformation Efficiency. Bioluminescence of Aequorea victoria. Transformation Efficiency.

131 views • 12 slides

Transformation Procedure Summary Incorporation of Foreign DNA Adhere DNA to Cells on ice Heat shock and DNA Entry Recove

Transformation Procedure Summary Incorporation of Foreign DNA Adhere DNA to Cells on ice Heat shock and DNA Entry Recovery on ice Plating Cells Screen for Transformants. 10 ul of pGlo (loopful of plasmid DNA) + one colony. 250 ul CaCl 2 (Transformation Solution) on ice for 10 min.

300 views • 10 slides

pGLO followup and term 4!!!

pGLO followup and term 4!!!. Announcements:. Before April Break you must schedule a 5 minute conference with me after school or at 7:45. List is on the board. This is a HW assignment to set goals for term 4! pGLO lab is due Friday 4/11

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Problem 36_1.1

Problem 36_1.1

92 views • 2 slides

Siemens Westinghouse Competition Math : Science : Technology National Finalist

Siemens Westinghouse Competition Math : Science : Technology National Finalist.

86 views • 1 slides