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Steady-State Enzyme Kinetics Scott Morrical smorrica@uvm B407 Given Bldg. General References:

Steady-State Enzyme Kinetics Scott Morrical smorrica@uvm.edu B407 Given Bldg. General References: Textbook: Frey & Hegeman, Chapter 2, pp. 69-128 Reserve: Cornish-Bowden (1979) Enzyme Kinetics , Butterworth & Co., London Websites: Dr. Peter Birch, University of Paisley

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The Meaning of Km & V

k 1. k 2. E + S ES E + P. k -1. The Meaning of Km & V Recall from the Briggs & Haldane derivation (Lecture 1) that for the 2-step reaction the velocity at steady-state is given by the equation v = k 2 [E t ][S] / ((k -1 + k 2 )/k 1 + [S]) = V[S] / (Km + [S])

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Steady-State Enzyme Kinetics Scott Morrical smorrica@uvm B407 Given Bldg. General References:

The Meaning of Km &amp; V

The Meaning of Km & V