'Taqman probe' presentation slideshows

Mei Baker, M.D., FACMG Associate Professor, Department of Pediatrics

Universal Newborn Screening for Severe Combined Immunodeficiency APHL/CDC Newborn Screening Molecular Workshop Atlanta, GA July 9, 2012. Mei Baker, M.D., FACMG Associate Professor, Department of Pediatrics Co-Director, NBS Laboratory at WSLH

461 views • 25 slides

qPCR

qPCR. Taylor Britton. Introduction. Quantitative polymerase chain reaction ( qPCR ) Monitor the amplification of a targeted DNA molecule during PCR in real-time. Limitations of PCR quantification. Poor precision Low sensitivity.

879 views • 14 slides

200

103 views • 1 slides

Supplementary Fig.1

A. Genomic DNA. Restriction endonuclease digestion. Fragmented genome. Hybridization with biotinylated probe targeting EGFR exon 20. EGFR exon 20 ssDNA isolated by Streptavidin Magnetic Beads. PNA-enriched TaqMan real-time PCR to detect T790M mutation. B. B.

125 views • 1 slides