'Southern blot' presentation slideshows

Recombinant DNA Technology

Recombinant DNA Technology. Site directed mutagenesis Genetics vs. Reverse Genetics Gene expression in bacteria and viruses Gene expression in yeast Genetic engineering in plants Genetic engineering in animals Gene therapy in mammals Detection of disease alleles.

1.07k views • 90 slides

Studying differences/similarities in Individuals

Studying differences/similarities in Individuals. Methods used to study differences between individuals RFLP SNP DNA Repeats. Genetic polymorphism. Genetic Polymorphism: A difference in DNA sequence among individuals, groups, or populations.

823 views • 59 slides

Transgenic cow research at AgResearch

Transgenic cow research at AgResearch. Liz Carpenter Dairy Biotechnology. Contents of the Talk. Introduction to AgResearch Terminology Steps to develop a transgenic Biological implications Ethical implications Questions. Introduction to AgResearch.

544 views • 42 slides

RFLP analysis

RFLP analysis. RFLP= Restriction fragment length polymorphism Refers to variation in restriction sites between individuals in a population These are extremely useful and valuable for geneticists (and lawyers) On average two individuals (humans) vary at 1 in 1000 bp

808 views • 40 slides

RENAL MITOCHONDRIAL DISEASES

486 views • 33 slides

Characterisation of filaggrin gene and results of mutation screening

Characterisation of filaggrin gene and results of mutation screening. Louise McClelland 13 th October 2010. Key words. Filaggrin Polyprotein profilaggrin Epidermal differentiation cDNA library Somatic hybrids M13 sequencing Long range PCR Ichthyosis vulgaris

895 views • 26 slides

MCB 7300: Southern Blot Lab

MCB 7300: Southern Blot Lab. Southern, Northern & Western Blotting Background Overview Detailed Protocol. Who is this guy?. Sir Edwin Mellor Southern of course (1938- ). Inventor of the Southern blot http://en.wikipedia.org/wiki/Edwin_Southern.

676 views • 26 slides

Molecular & Genomic Surgery

Molecular & Genomic Surgery. Eric M. Wilson 1/5/10. DISCLAIMER. I am by no means an expert on this subject. If you have questions, I suggest you ask Tom Theruvath or look it up. . Molecular Biology. DNA. DNA Replication. Gene Expression. Transcription.

442 views • 24 slides

DNA fingerprinting

DNA fingerprinting. Look at the DNA of the mother, father and child Could these parents produce this child?. DNA fingerprinting is used to determine paternity. DNA fingerprinting. 2 methods Southern Blot and Restriction fragment length polymorphism PCR and VNTRs. Different types of DNA.

630 views • 23 slides

SOUTHERN BLOTTING

SOUTHERN BLOTTING. The technique was developed by E.M. Southern in 1975. The Southern blot is used to detect the presence of a particular piece of DNA in a sample. The DNA detected can be a single gene, or it can be part of a larger piece of DNA such as a viral genome. SOUTHERN BLOTTING.

1.73k views • 21 slides

chapters 10 & 11 taxonomy, phylogenetics and prokaryotic diversity

chapters 10 & 11 taxonomy, phylogenetics and prokaryotic diversity. organism grouping. identification binomial nomenclature classification & phylogenetics organism similarity. 16S rDNA sequence analysis & phylogeny. identification: biochemical testing.

411 views • 19 slides

Louise Stanley

363 views • 19 slides

PCR, Gel Electrophoresis, and Southern Blotting

PCR, Gel Electrophoresis, and Southern Blotting. By: D.D. What Is PCR?. Stands for Polymerase Chain Reaction.

830 views • 17 slides

Myotonic Dystrophy

560 views • 16 slides

Southern Blotting

Southern Blotting . General Scheme for Southern Blot. Restriction Digest. Gel Electrophoresis . DNA Preparation: Denaturation/Depurination. Transfer to filter: Blotting. Detecting DNA: Probing. Southern Blot Analysis. Southern Blot. done. Restriction Digest of DNA Electrophoresis

2.49k views • 15 slides

SOUTHERN BLOTTING

SOUTHERN BLOTTING. Presented by: Imran Fakhroni Nurkholis Nugroho Nino Radiansyah Indra Ardi Fauzi Arfita Sari. SOUTHERN BLOTTING. The technique was developed by E.M. Southern in 1975. The Southern blot is used to detect the presence of a particular piece of DNA in a sample.

796 views • 15 slides

Fragile X Syndrome - Clinical

480 views • 15 slides

Genotyping A Transgenic Mouse INK4A Knock-out & INK4A Wild-type

251 views • 13 slides

Aim: To understand how the olfactory transduction system is organized

168 views • 9 slides

UVM11

107 views • 1 slides