'Sequencing reaction' presentation slideshows

SEQUENCING DNA Jos. J. Schall Biology Department University of Vermont

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HUMAN GENOME PROJECT

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Today

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Why bother with a new technology?

Why bother with a new technology?. Current (2005) $1.00 per kilobase 20 bp / second 100 Gb / year. NHGRI Goals $ ? 100x, 5yrs $ ? 10,000x, 15yrs. Collins, Morgan, Patrinos (2003). Growth of GenBank. 60. 50. 40. 30. Base pairs of DNA (billions). Sequences (millions). 20. 10. 0.

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Today

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DNA Sequencing

DNA Sequencing. Determining DNA Sequence. Originally 2 methods were invented around 1976, but only one is widely used: the chain-termination method invented by Fred Sanger.

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5. DNA Sequencing

5. DNA Sequencing. pp 379 - 381. Fredrick Sanger. Developed dideoxy termination sequencing method Nobel Laureate, 1980 Nobel Laureate, 1958; determined insulin sequence. DNA Sequencing. The components of a sequencing reaction are:. dideoxyribose when both oxygen atoms removed.

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NAC Family Genes AT1G01720 AT1G77450

NAC Family Genes AT1G01720 AT1G77450. Yosuke Ishida From Kyoto Japan. Basic information on my genes. Gene 1. Gene 2. AT1G01720. AT1G77450. 1.2kbp. 1.1kbp. 1 142 302 388 666 754 1186 1490. 1 76 245 347 622 711 1029 1308. 1129. 484. T-DNA. T-DNA.

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