'Secondary antibody' presentation slideshows

Fluorescence and Confocal Microscopy

Fluorescence and Confocal Microscopy. Yvona Ward Cell and Cancer Biology Branch. OUTLINE. Immunofluorescent Staining Conventional Fluorescent Microscopy Confocal Microscopy Applications. Immunofluorescent Staining. Immunofluorescent staining makes use of antibodies to locate and

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Cells

Methods in cell biology and functional genomics overview. Individualized. High throughput. FACS. Cells. DNA. FACS. Genomics. Markers. RNA. Tissues. Transcriptomics. In situ hyb. Protein. Organs. Immuno techniques. Proteomics. Metabolite. Organisms. Microscopy. Metabolomics.

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Antigen antibody reactions

Antigen antibody reactions. Enzyme linked immunosorbant assay (ELISA). The method depends on conjugation of an enzyme to either Ag or Ab , then substrate is added as a quantitative measure of enzyme activity. There are 3 ways of ELIZA testing: Indirect method. Direct or Double antibody

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Novel Approach to Probe Subunit-specific Contributions to NMDAR Trafficking

Novel Approach to Probe Subunit-specific Contributions to NMDAR Trafficking. Introduction. NR2A sorts predominantly into the degradation pathway, and NR2B preferentially traffics through the recycling pathway NR2A/NR2B heterodimers traffic preferentially through the recycling pathway

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Immunoglobulins Scan pp. 221-228 for background, but you are not responsible for this material.

Immunoglobulins Scan pp. 221-228 for background, but you are not responsible for this material. You are responsible for the material on antibodies, pp. 228-233, and the rest of the chapter. Focus on protein-ligand binding

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Laboratory procedure that allows you to:

Western Analysis. Laboratory procedure that allows you to:. 1. Verify the expression of a protein. 2. Determine the relative amount of a protein present in different samples. 3. Analyze protein-protein interactions. Two Main Types of Westerns. 1. Denaturing (Most Commonly Used).

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Laboratory procedure that allows you to:

Western Analysis. Laboratory procedure that allows you to:. 1. Verify the expression of a protein. 2. Determine the relative amount of a protein present in different samples. 3. Analyze protein-protein interactions. Two Main Types of Westerns. 1. Denaturing (Most Commonly Used).

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Cell Separation Using Magnetic Beads

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Western Blotting

Lab#6. Western Blotting. BCH 462[practical]. Objective : Western blotting of proteins from SDS-PAGE. . Western blot:. -Is used to identify specific antigens[proteins], based on their ability[the antigens] to bind to antibodies. - sometimes called the protein immunoblot Principle:

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Antibody Detection

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High content screening workflow

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Na + /H + exchanger regulatory factor 1 (NHERF1) and angiogenesis in familial breast cancer

FIRENZE 7- 9 Settembre 2009. Na + /H + exchanger regulatory factor 1 (NHERF1) and angiogenesis in familial breast cancer A Mangia*, A Malfettone*, C Salvatore**, B Stea*, G Simone** and A Paradiso* *Clinical Experimental Oncology Laboratory, National Cancer Institute-Bari

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Research Techniques Made Simple: Enzyme Immunoassay and ELISA

Research Techniques Made Simple: Enzyme Immunoassay and ELISA. Stephanie D. Gan 1 and Kruti R. Patel 2 1 Department of Dermatology and 2 Program in Molecular and Translational Medicine, Department of Medicine, Boston University and Boston Medical Center. What is EIA/ELISA?.

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Western Blotting

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293T

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Supplementary Figure 1: Flavivirus immune status of donors

Supplementary Figure 1: Flavivirus immune status of donors.

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Tissue Fixation

IHC Staining Flow Chart. Tissue Fixation. Antigen Retrieval. Staining. Chromogen (DAB). Secondary Antibody (HRP-DAB). Primary Antibody. Counterstain.

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Target

Nanosphere. Receptors (secondary antibody). Target. QDs. Active sites. Receptors (antibody, target receptors). Microsphere.

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Supplementary Figure S1

Supplementary Figure S1. T-FGM AhR+/+. Transmitted. DAPI. Alexa 488. 2 0 m m. 2 0 m m. 2 0 m m.

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