100+ Ribosome profiling PowerPoint (PPT) Presentations, Ribosome profiling PPTs

Ribosome profiling - PowerPoint PPT Presentation

Figure S1. Ribosome profiling library preparation and characterization

Supplementary Figures, Jensen et al. Units RNase/OD extract. A. B. MgCl 2. TRIzol. EDTA. 0. 1. 4. 15. 30. 60. 100 nt. 100 nt. 30 nt. 30 nt. 20 nt. 20 nt. ? -tubulin. ? -tubulin. C. mRNA. Ribosome. 1. 2. 3. Percent of reads for each position within the codon.

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Ribosome Profiling

CD genomics provides a ribosome-profiling strategy that is based on the deep sequencing of ribosome-protected mRNA fragments and enables genome-wide investigation of translation with sub cordon resolution. Using nuclease digestion, the ribosome position and the translated message can be precisely determined by analyzing the protected ~30-nt area of the mRNA template.

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58 views • 3 slides

Ribosome profiling

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Ribosome profiling - PowerPoint PPT Presentation

Figure S1. Ribosome profiling library preparation and characterization

Ribosome Profiling

Ribosome profiling