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Read length 150 bp - PowerPoint PPT Presentation

Sequencing technology and assembly

Sequencing technology and assembly. Sanger sequencing. Sanger sequencing with radioactivity High throughput Sanger sequencing with fluorescence. Roche/454 sequencing. Yield: 500,000,000 bp Cost: $5,000 Time: ~1 min per bp Read length: 450 bp - > 1kb. Pyrosequencing. Illumina sequencing.

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Read length 150 bp - PowerPoint PPT Presentation

Sequencing technology and assembly