'Protein corona' presentation slideshows

Prof. Kenneth Dawson Centre for BioNano Interactions

466 views • 33 slides

EpitopeMap RNP Mid-term Review

EpitopeMap RNP Mid-term Review. 14 th October 2010 Dr. Iseult Lynch University College Dublin. Significant progress in understanding role of protein corona during EpitopeMap. Outside cell. Inside cell. CHEMICALS PARTITION NANOPARTICLES TAKEN UP.

234 views • 13 slides

Formation of Protein Corona on Rhodium Citrate-Functionalized Magnetic Nanoparticles and Their Interaction with Human Ma

Nanoscience and Nanotechnology enable innovations for Medicine and for Biomedical Science. Among the current nanoparticles, maghemite associated with rhodium citrate (Magh-RhCit) has been shown to be promising, because it reduces side effects of drugs while maintaining cytotoxicity for tumor cells. However, NPs in contact with biological fluids are immediately coated by proteins (protein corona) that are unique to each nanomaterial. In this study, the adsorption of the most abundant binding proteins was studied in vitro using a three-step analysis: (1) characterization of the magnetic fluid (Magh-RhCit) before and after incubation with serum; (2) identification and physical and biochemical analysis of protein corona; and (3) the cellular internalization of these nanoparticles in human macrophages.

147 views • 13 slides