'Primer sequence' presentation slideshows

Primer Design & Restriction Analysis 2 nd April 2014

Primer Design & Restriction Analysis 2 nd April 2014. Carrie Iwema , PhD, MLS, AHIP Information Specialist in Molecular Biology Health Sciences Library System University of Pittsburgh iwema@pitt.edu http://www.hsls.pitt.edu/molbio. Goals:. PCR primer construction & analysis

1.38k views • 111 slides

Primer Design & Restriction Analysis 10 th April 2013

Primer Design & Restriction Analysis 10 th April 2013. Carrie Iwema , PhD, MLS, AHIP Information Specialist in Molecular Biology Health Sciences Library System University of Pittsburgh iwema@pitt.edu http://www.hsls.pitt.edu/molbio. Goals:. PCR primer construction & analysis

1.51k views • 111 slides

Cyclosporiasis: An Emerging Zoonotic Disease?

Cyclosporiasis: An Emerging Zoonotic Disease? . Proposed Life Cycle. Ingested oocysts excyst in GI tract Sporozoites invade small intestinal epithelial cells Asexual forms 2 different sized merozoites type I meronts (8-12 merozoites) type II meronts (4 merozoites) Sexual forms

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Molecular Methods in Bioremediation

Molecular Methods in Bioremediation Polymerase Chain Reaction (PCR) PCR Machine Gel Electrophoresis Primer Primer sequence Annealing Size Reference temperatures ( C) 16S rDNA universal-F 5 ? -CCGTCGACAGAGTTTGATCCTGGCTCAG- 3 ? 55 1500 1 16S rDNA universal-R

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Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR). Developed in 1983 by Kary Mullis Major breakthrough in Molecular Biology Allows for the amplification of specific DNA fragments from genome or genetic sample of an organism Benefit is that only a small amount of sample DNA needed.

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501 views • 17 slides

Some suggestion for KO and complementation

Some suggestion for KO and complementation. Chengkang Zhang Aug.17 ? 2010. KO vector. DO we need the KO vector? NO, we just need the AHB fragment, OR AH&HB fragments. SOE-PCR. 1. ????.

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KHJDGP002~003 PCR report

2008. 09. 29. KHJDGP002~003 PCR report. 1. Sample check. 1-1) KHJDGP002 (21ea ?? check). 100ng 1kb 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21. Sample 1ul loading. 2008. 09. 29.

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Figure S1

A. B. C. Figure S1.

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Supplementary Table1. The primer sequence for amplification of each transcripts.

Supplementary Table1. The primer sequence for amplification of each transcripts.

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InfA_WT

Protein Expression. kindly provided by Dr. Shin-Ru Shih. A/WSN/33/(H1N1): InfA_WT. E186A-W187S mutation by single primer. Primer sequence:. GAATGG ggtcctcatcggaggactt GCTAGC aataataacacagttcgagtctctga. -InfA_E186AW-187S. -InfA_WT. pCR3.1. InfA_WT.

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