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Practical tips for cloning, expressing and purifying proteins for structural biology

Practical tips for cloning, expressing and purifying proteins for structural biology

Practical tips for cloning, expressing and purifying proteins for structural biology. Aled Edwards Banting and Best Department of Medical Research University of Toronto, Canada aled.edwards@utoronto.ca Affinium Pharmaceuticals Toronto, Canada aedwards@afnm.com.

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658 views • 56 slides
A Novel Multigene Family May Encode Odorant Receptors: A Molecular Basis for Odor Recognition

A Novel Multigene Family May Encode Odorant Receptors: A Molecular Basis for Odor Recognition

A Novel Multigene Family May Encode Odorant Receptors: A Molecular Basis for Odor Recognition. Linda Buck and Richard Axel Published in Cell, Volume 65, 175-187 April 5, 1991. Presented by Adam Warner on Oct. 12, 2004. Linda Buck. Research Associate Assistant Investigator . 1984-1991.

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419 views • 27 slides
PROTEIN EXPRESSION IN MAMMALIAN CELLS ~ Techniques Workshop 23 May 2007

PROTEIN EXPRESSION IN MAMMALIAN CELLS ~ Techniques Workshop 23 May 2007

PROTEIN EXPRESSION IN MAMMALIAN CELLS ~ Techniques Workshop 23 May 2007. EXTRACELLULAR PROTEINS Large extracellular domains Modular multidomain organisation Posttranslational modifications Disulfide bridges Glycosylation. INTEGRIN ? V ? 3 9 domains 28 disulfide bridges

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877 views • 18 slides
RPCI-11 BES Pairs to Human Genome

RPCI-11 BES Pairs to Human Genome

RPCI-11 BES Pairs to Human Genome. Question how many RPCI-11 BES pairs can be placed on human genome? RPCI-11 BES Pairs Q20 bases >=350 bp, false negative by low quality sequences Unique bases >= 100 bp, false negative by repeats masking 30,030 BESs from 15,015 clones

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117 views • 5 slides

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