'Pol ii' presentation slideshows

Chapter 31

Chapter 31. Transcription and Regulation of Gene Expression to accompany Biochemistry, 2/e by Reginald Garrett and Charles Grisham.

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Introduction to Transcriptional Machinery

Introduction to Transcriptional Machinery. "DNA makes RNA, RNA makes protein, and proteins make us." Francis Crick. Central Dogma of Molecular Biology. RNA Polymerase of E. Coli. Transcribes all mRNA, rRNAs and tRNAs 7,000 molecules per cell

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Post-transcriptional gene control

Post-transcriptional gene control. Subjects, covered in the lecture. Processing of eukaryotic pre-mRNA - capping -polyadenylation -splicing -editing Nuclear transport. Processing of eukaryotic pre-mRNA : the classical texbook picture.

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Chapter Eleven Transcription of the Genetic Code: The Biosynthesis of RNA

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Everything you wanted to know about ENCODE

Everything you wanted to know about ENCODE . But were afraid to ask. Top 5 Reasons Biologists Go Into Bioinformatics. 5 - Microscopes and biochemistry are so 20th century. Top 5 Reasons Biologists Go Into Bioinformatics. 5 - Microscopes and biochemistry are so 20th century.

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Everything you wanted to know about ENCODE

Everything you wanted to know about ENCODE . But were afraid to ask. Top 5 Reasons Biologists Go Into Bioinformatics. 5 - Microscopes and biochemistry are so 20th century. Top 5 Reasons Biologists Go Into Bioinformatics. 5 - Microscopes and biochemistry are so 20th century.

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Gene Expression

Gene Expression. 1: Activation (overview), Transcription, Translation. ? The Goodwin Equations. Review. An active gene is one that is being transcribed , and whose transcription product is being translated , and whose translation product affects cellular behavior/development.

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DNA Replication Ch 20 and 21

DNA Replication Ch 20 and 21. Review session tomorrow at 5 pm and Monday at 5 pm Office hours by appointment next week DNA replication. Meselson and Stahl. Semidiscontinuous vs. Continuous vs. Discontinuous. Semidiscontinuous Replication. Leading strand Replicated continuously

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Molecular Genetics Bio350/516 Lecture 17, 3/28/06

Molecular Genetics Bio350/516 Lecture 17, 3/28/06. I. Chapter 10 (continued) A. Promoters 1. Pol II (class II) a. Linker scanning 2. Pol I (class I) 3. Pol III (class III) B. Enhancers and Silencers . Class II Promoters: mRNA genes.

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Exon selection factor

Overview of mRNA Splicing. Factors such as U1 and U2 snRNP identify splice sites. Exon selection factor. Exon selection factor. U1 snRNP. U2 snRNP. Exon 1. AGGU. Intron 1. A AGG. Exon 2.

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DNA: The genetic material

DNA: The genetic material. Mendel did not know about DNA or chromosomes!. Experiments that proved DNA is heritable material 1928: transformation (Griffiths). Avery, MacLeod, and McCarty:DNA is the transforming molecule (1944). Used same Streptococcus strains as Griffiths

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DNA: The genetic material

DNA: The genetic material. Mendel did not know about DNA or chromosomes!. Experiments that proved DNA is heritable material 1928: transformation (Griffiths). Avery, MacLeod, and McCarty:DNA is the transforming molecule (1944). Used same Streptococcus strains as Griffiths

389 views • 23 slides

Transcription and gene regulation

Transcription and gene regulation. sven.nelander@wlab.gu.se. RNA in a mammalian cell. Amount #genes Protein-coding RNA 5% 20000 (mRNA) Non-coding RNA 95% <1000 (tRNA, rRNA, miRNA). mRNA expression levels. X axis: organ (skin, heart, brain and so on)

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Announcements

Announcements. Grading of Mendel papers: A= completeness of answer (40); F = flow and organization (15); R = references (10); S = spelling and grammar (10).

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4.21 Prokaryotic Genetics

4.21 Prokaryotic Genetics. By Lance Goodridge and Devin Callanan. Defining Prokaryotes. Unicellular organisms Lacks cell nucleus Lacks mitochondria + chloroplasts. Defining Prokaryotes 2. Very small Live near water Extremophiles. Evolution of Prokaryotes. Organized into two kingdoms

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LECTURE 17: RNA TRANSCRIPTION, PROCESSING, TURNOVER

LECTURE 17: RNA TRANSCRIPTION, PROCESSING, TURNOVER. Levels of specific messenger RNAs can differ in different types of cells and at different times in the same cell. Control of RNA abundance can be at the level of transcription initiation, transcription elongation, processing or degradation.

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ChIP-chip Data

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4 male, 4 female LCLs

Figure S1. An illustration of the study design. 3 Species. Human. Chimpanzee. Rhesus Macaque. 8 Samples per species. 5 male, 3 female LCLs. 4 male, 4 female LCLs. 6 male, 2 female LCLs. Expression: RNAseq. Active Gene Marks: Pol II ( ChIPseq ) H3K4me3 ( ChIPseq ).

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The Nucleus

277 views • 16 slides