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Lecture 8: Mass Spectrometry, Applications

Lecture 8: Mass Spectrometry, Applications

Lecture 8: Mass Spectrometry, Applications. Last week we saw how we do Mass Spectrometry, now we will see why we do mass spectrometry. Mass Spec is a technique which, when you take a mixture of stuff, nearly everything in that mixture will give you an independent signal.

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360 views • 33 slides
Problem

Problem

Data Processing Algorithms for Analysis of High Resolution MSMS Spectra of Peptides with Complex Patterns of Posttranslational Modifications Shenheng Guan and Alma L. Burlingame. Problem. Input: An MS/MS spectrum of a mixture of peptides: Heavily modified protein Same amino acid sequence

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406 views • 27 slides
Proteomics: 2D Gel

Proteomics: 2D Gel

Proteomics: 2D Gel. Proteome analysis ?? (2D). Root Proteome. Nodule Proteome. Table I. Proteins identified in soybean root and nodule through MALDI-TOF/MS & LC-MS/MS. ???? ?? ????. ??. ?????. MS spectrum. ???. Protein database. Peptide sequences. Protein id. A. B. A. B.

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516 views • 10 slides
Yat Li Department of Chemistry & Biochemistry University of California, Santa Cruz

Yat Li Department of Chemistry & Biochemistry University of California, Santa Cruz

CHEM 146C_Experiment #5 Measuring Gas-phase Basicities of Amino Acids using an Ion Trap Mass Spectrometer. Yat Li Department of Chemistry & Biochemistry University of California, Santa Cruz. Objective. In this laboratory experiment, we will learn:. The principle of mass spectrometry (MS)

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189 views • 9 slides
Data independent acquisition methods for metabolomics

Data independent acquisition methods for metabolomics

Data independent acquisition methods for metabolomics. Stephen Tate, Ron Bonner AB SCIEX, 71 Four Valley Drive, Concord, ON, L4K 4V8 Canada. MS/MS spectrum, 300 - 325. INTRODUCTION Data independent analysis collects MSMS Spectra of all ions without prior information of parent molecules

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290 views • 1 slides

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