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Mrna seq - PowerPoint PPT Presentation

The BCM Microarray Core Facility: Closing the Next Generation Gap

Isolate mRNA and Fragment. 1-10 m g of Total RNA. T. A. A. T. 1st and 2nd strand cDNA synthesis. Ligate Adapters. Sequencing on GAIIx. Enriched cDNA Library by PCR. Cluster Generation on Flow Cell. Size selection and gel purification.

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Mrna seq - PowerPoint PPT Presentation

The BCM Microarray Core Facility: Closing the Next Generation Gap