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Long chromatin rna - PowerPoint PPT Presentation

$Cell growth, fractionation, RNA isolation$

Cell growth, fractionation, RNA isolation

Cell growth, fractionation, RNA isolation. Cell growth conditions. Growth to 600,000-800,000 cells/ml Media RPMI1640 + 10% FBS + pen-strep Subcellular fractionation done on fresh cells Typically 50-70% of cells in S-phase as assessed by the FACS analysis. Cyto-nuclear fractionation.

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Long chromatin rna - PowerPoint PPT Presentation

Cell growth, fractionation, RNA isolation